BAZ2B antibody (C-Term)

Catalog No. ABIN374295

The Goat Polyclonal anti-BAZ2B antibody (ABIN374295) specifically detects BAZ2B in WB and IHC (p). The antibody is reactive with Human, Mouse, Cow and Rat samples.

Quick Overview for BAZ2B antibody (C-Term) (ABIN374295)

Target: BAZ2B (Bromodomain Adjacent To Zinc Finger Domain, 2B (BAZ2B))

Reactivity: Human, Mouse, Cow, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This BAZ2B antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-BAZ2B Antibody

Binding Specificity: C-Term

Sequence: C-YFEKKWTDTF KVS

Specificity: This antibody detects BAZ2B at C-term.

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat, CowSpecies reactivity (tested):Human

Purification: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogen: Peptide from C Terminus of the protein sequence according to NP_038478.2 Genename: BAZ2B

Application Details

Application Notes: Peptide ELISA: Limit dilution 1: 4000. Western blot: Preliminary experiments gave an approx 35 kDa band in Testis and Brainlysates after 0.3 μg/mL antibody staining. Please note that currently we cannot find anexplanation in the literature for the band we observe given the calculated size of 225 kDaaccording to NP_038478. The 35 kDa band was successfully blocked by incubation with theimmunizing peptide. We would appreciate any feedback from people in the field - have anyresults been reported with other antibodies/lysates? Have any further splicevariants/modified forms been reported?Immunohistochemistry: 1-2 μg/mL. In paraffin embedded Human Small Intestine showsprimarily nuclear staining in a number of cells of the lamina propria.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.

Target Details for BAZ2B

Target: BAZ2B (Bromodomain Adjacent To Zinc Finger Domain, 2B (BAZ2B))

Alternative Name: BAZ2B

Background: BAZ2B may play a role in transcriptional regulation interacting with ISWI. It is expressed at varying levels in several tissues, whereas a smaller transcript was expressed specifically in testis.Synonyms: Bromodomain adjacent to zinc finger domain protein 2B, KIAA1476, hWALp4

Gene ID: 29994

NCBI Accession: NP_038478

UniProt: Q9UIF8