KAP1 antibody (C-Term)

Catalog No. ABIN374296

This Goat Polyclonal antibody specifically detects KAP1 in WB, IF, IHC (p), EIA and ChIP. It exhibits reactivity toward Human and Mouse. It has been mentioned in 2+ publications

Quick Overview for KAP1 antibody (C-Term) (ABIN374296)

Target: KAP1 (TRIM28) (Tripartite Motif Containing 28 (TRIM28))

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This KAP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA), Chromatin Immunoprecipitation (ChIP)

Product Details anti-KAP1 Antibody

Binding Specificity: C-Term

Sequence: C-SSQELSGGPG DGP

Specificity: This antibody detects TIF1-beta / TRIM28 at C-term.

Cross-Reactivity (Details): Species reactivity (tested):Human and Mouse.

Purification: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide

Immunogen: Synthetic peptide from the C-Terminus sequence according to NP_005753.1 Genename: TRIM28

Application Details

Application Notes: Peptide ELISA: Limit Dilution: 1/8000. Western blot: 0.2-1 μg/mL. Approx 100-120 kDa band seen in HepG2 lysate. Immunohistochemistry: 5 μg/mL (Ssuccessfully used on Human gastric cancer surgical

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-KAP1 antibody (ABIN374296)

Santanelli, Paolini, Longo: "Comments on: Improving breast cancer surgery: a classification and quadrant per quadrant atlas for oncoplastic surgery (Clough KB, Kaufman GJ, et al. ASO DOI 10.1245/s10434-009-0792-y)." in: Annals of surgical oncology, Vol. 18 Suppl 3, pp. S257-8; author reply S259-60, (2011) (PubMed).

Yokoe, Toiyama, Okugawa, Tanaka, Ohi, Inoue, Mohri, Miki, Kusunoki: "KAP1 is associated with peritoneal carcinomatosis in gastric cancer." in: Annals of surgical oncology, Vol. 17, Issue 3, pp. 821-8, (2010) (PubMed).

Target Details for KAP1

Target: KAP1 (TRIM28) (Tripartite Motif Containing 28 (TRIM28))

Alternative Name: TIF1-beta / TRIM28

Background: The KRAB (Kruppel Associated Box) domain is minimally about 45 amino acids in length and is a transcriptional repression domain found in numerous transcription factors. Over 220 KRAB zinc finger protein (KRAB ZFP) genes have been identified in the human genome. These proteins functionally repress transcription via specific interactions with KAP1 (KRAB Associated Protein 1). KAP1 is an 835 amino acid polypeptide that contains a RING finger, B boxes, and a PHD finger. KAP1 has been shown to form complexes with KRAB domain transcription factors and increase the efficiency with which they mediate repression. KAP1 has also been shown to directly interact with HP1 (heterochromatin protein 1) and KRAZ1 (Kruppel associated box containing zinc finger protein 1). KAP1 directly targets KRAZ1 to the foci of centromeric heterochromatin containing HP1alpha, helping to regulate transcriptional repression. Recent studies have shown that KAP1 mutants with the ability to bind KRAB but unable to bind HP1 leads to random distribution of KRAZ1 and strong transcriptional activation.Synonyms: KAP-1, KAP1, KRAB-associated protein 1, KRAB-interacting protein 1, KRIP-1, KRIP1, Nuclear corepressor KAP-1, RING finger protein 96, RNF96, TIF1 beta, TIF1B, Transcription intermediary factor 1-beta, Tripartite motif-containing protein 28

Gene ID: 10155

NCBI Accession: NP_005753

UniProt: Q13263

Pathways: Hedgehog Signaling, Positive Regulation of Response to DNA Damage Stimulus