FBXL2 antibody (N-Term)

Catalog No. ABIN374345

The Goat Polyclonal anti-FBXL2 antibody is suitable to detect FBXL2 in samples from Human, Rat, Cow and Dog. It has been validated for WB.

Quick Overview for FBXL2 antibody (N-Term) (ABIN374345)

Target: FBXL2 (F-Box and Leucine-Rich Repeat Protein 2 (FBXL2))

Reactivity: Human, Rat, Cow, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This FBXL2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-FBXL2 Antibody

Binding Specificity: N-Term

Sequence: VFSNNDEGLI NKK-C

Specificity: This antibody detects FBXL2 / FBL2 / FBL3 at N-term.

Cross-Reactivity (Details): Species reactivity (expected):Rat, Dog, CowSpecies reactivity (tested):Human

Purification: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogen: Peptide from N Terminus of the protein sequence according to NP_036289.2 Genename: FBXL2

Application Details

Application Notes: Peptide ELISA: Limit dilution 1: 32000. Western blot: 0.3-1.0 μg/mL. Approx 48 kDa band observed in Human Brain lysates(calculated MW of 47.8 kDa according to NP_036289.2).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3, 0.02 % sodium azide, 0.5 % bovine serum albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.

Target Details for FBXL2

Target: FBXL2 (F-Box and Leucine-Rich Repeat Protein 2 (FBXL2))

Alternative Name: FBXL2 / FBL2 / FBL3

Background: FBXL2 is a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination.Synonyms: F-box and leucine-rich repeat protein 2, F-box protein FBL2/FBL3, F-box/LRR-repeat protein 2

Gene ID: 25827

NCBI Accession: NP_036289

UniProt: Q9UKC9