GRAF antibody (C-Term)

Catalog No. ABIN374371

This Goat Polyclonal antibody specifically detects GRAF in WB. It exhibits reactivity toward Human, Mouse, Rat, Cow and Dog.

Quick Overview for GRAF antibody (C-Term) (ABIN374371)

Target: GRAF (ARHGAP26) (rho GTPase Activating Protein 26 (ARHGAP26))

Reactivity: Human, Mouse, Rat, Cow, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This GRAF antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-GRAF Antibody

Binding Specificity: C-Term

Sequence: C-KTGLIPENYV EFL

Specificity: This antibody detects ARHGAP26/OPHN1L at C-term. It is expected to recognise both reported isoforms.

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat, Canine (Dog) and Bovine (Cow).
Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide

Immunogen: Peptide from C-Terminus of the protein sequence according to NP_055886.1, NP_001129080.1. Genename: ARHGAP26

Application Details

Application Notes: Peptide ELISA: Limit dilution 1/64000. Western blot: 0.3-1 μg/mL. Approx 90 kDa band observed in Human Heart lysates(calculated MW of 92.0 kDa according to NP_055886.1). An additional strong band ofunknown identity was also consistently observed at 10 kDa. This band was successfullyblocked by incubation with the immunising peptide. We would appreciate any feedbackfrom people in the field - have any results been reported with other antibodies/lysates?Have any further splice variants/modified forms been reported?
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 with 0.02 % Sodium Azide and 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C.

Target Details for GRAF

Target: GRAF (ARHGAP26) (rho GTPase Activating Protein 26 (ARHGAP26))

Alternative Name: ARHGAP26 / OPHN1L

Background: Synonyms: GRAF, GTPase regulator associated with focal adhesion kinase, KIAA0621, Oligophrenin-1-like protein, Rho GTPase-activating protein 26, Rho-type GTPase-activating protein 26

Gene ID: 23092

NCBI Accession: NP_055886

UniProt: Q9UNA1