KPNA3 antibody (C-Term)

Catalog No. ABIN374393

Product Details anti-KPNA3 Antibody

Target: KPNA3 (Karyopherin alpha 3 (Importin alpha 4) (KPNA3))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat, Cow, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This KPNA3 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Sequence: C-DPTANLQTKE FNF

Specificity: This antibody detects Importin subunit alpha-3 at C-term.

Cross-Reactivity (Details): Species reactivity (expected):Canine (Dog), Cow.
Species reactivity (tested):Human, Mouse, Rat.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide

Immunogen: Peptide from C Terminus of the protein sequence according to NP_002258.2 Genename: KPNA3

Application Details

Application Notes: Peptide ELISA: Limit dilution 1: 32000. Western blot: 0.1-0.5 μg/mL. Approx 55-60 kDa band observed in Human Testis and Kidneylysates (predicted size of approx.50 kDa according to NP_002258). Immunohistochemistry: 5-10 μg/mL. In paraffin embedded Human Heart shows strong pixulate staining ofmyocardial fibres in transverse section.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-KPNA3 antibody (ABIN374393)

Silva, Martins, Tavares, Morgado: "Inhibition of spinal 5-HT3R reverted diabetes-induced mechanical hypersensitivity in a GABAAR-mediated neurotransmission-dependent manner." in: Neuroscience, Vol. 304, pp. 228-39, (2016) (PubMed).

Méndez-Lucas, Duarte, Sunny, Satapati, He, Fu, Bermúdez, Burgess, Perales: "PEPCK-M expression in mouse liver potentiates, not replaces, PEPCK-C mediated gluconeogenesis." in: Journal of hepatology, Vol. 59, Issue 1, pp. 105-13, (2013) (PubMed).

Ohara, Hata, Yasuhara, Mehmood, Yoneda, Nakagawa, Yamashita: "Axotomy induces axonogenesis in hippocampal neurons by a mechanism dependent on importin ?." in: Biochemical and biophysical research communications, Vol. 405, Issue 4, pp. 697-702, (2011) (PubMed).

Huenniger, Krämer, Soom, Chang, Köhler, Depping, Kehlenbach, Kaether: "Notch1 signaling is mediated by importins alpha 3, 4, and 7." in: Cellular and molecular life sciences : CMLS, Vol. 67, Issue 18, pp. 3187-96, (2010) (PubMed).

Target Details for KPNA3

Target: KPNA3 (Karyopherin alpha 3 (Importin alpha 4) (KPNA3))

Alternative Name: KPNA3 / Importin alpha-3 (KPNA3 Products)

Synonyms: IPOA4 antibody, si:dz79f24.2 antibody, importin antibody, SRP1 antibody, SRP1gamma antibody, SRP4 antibody, hSRP1 antibody, ipoa4 antibody, srp1 antibody, srp1gamma antibody, srp4 antibody, karyopherin (importin) alpha 3 antibody, karyopherin alpha 3 (importin alpha 4) antibody, karyopherin subunit alpha 3 antibody, karyopherin alpha 3 (importin alpha 4) L homeolog antibody, Kpna3 antibody, kpna3 antibody, KPNA3 antibody, kpna3.L antibody

Background: The transport of molecules between the nucleus and the cytoplasm in eukaryotic cells is mediated by the nuclear pore complex (NPC) which consists of 60-100 proteins and is probably 120 million daltons in molecular size. Small molecules (up to 70 kD) can pass through the nuclear pore by nonselective diffusion, larger molecules are transported by an active process. Most nuclear proteins contain short basic amino acid sequences known as nuclear localization signals (NLSs). KPNA3, encodes a protein similar to certain nuclear transport proteins of Xenopus and human. The predicted amino acid sequence shows similarity to Xenopus importin, yeast SRP1, and human RCH1 (KPNA2), respectively. The similarities among these proteins suggests that KPNA3 may be involved in the nuclear transport system. The transport of molecules between the nucleus and the cytoplasm in eukaryotic cells is mediated by the nuclear pore complex (NPC) which consists of 60-100 proteins and is probably 120 million daltons in molecular size. Small molecules (up to 70 kD) can pass through the nuclear pore by nonselective diffusion, larger molecules are transported by an active process. Most nuclear proteins contain short basic amino acid sequences known as nuclear localization signals (NLSs). KPNA3, encodes a protein similar to certain nuclear transport proteins of Xenopus and human. The predicted amino acid sequence shows similarity to Xenopus importin, yeast SRP1, and human RCH1 (KPNA2), respectively. The similarities among these proteins suggests that karyopherin alpha-3 may be involved in the nuclear transport system.Synonyms: Importin alpha Q2, Karyopherin subunit alpha-3, QIP2, SRP1-gamma

Gene ID: 3839

NCBI Accession: NP_002258

UniProt: O00505

Pathways: Protein targeting to Nucleus