SIPA1 antibody (C-Term)

Catalog No. ABIN374401

Product Details anti-SIPA1 Antibody

Target: SIPA1 (Signal-Induced Proliferation-Associated 1 (SIPA1))

Binding Specificity: C-Term

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This SIPA1 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: C-ASKQLGSPTA DLA

Specificity: This antibody detects SPA1 / SIPA1 at C-term.

Cross-Reactivity (Details): Species reactivity (tested):Human

Purification: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogen: Peptide from C Terminus of the protein sequence according to NP_694985.28, NP_006738.3 Genename: SIPA1

Application Details

Application Notes: Peptide ELISA: Limit dilution 1: 32000. Western blot: 1-3 μg/mL. Approx.150 kDa band observed in Human lysates ofEBV-immortilized lymphoblastoid (calculated MW of 110 kDa according to NP_694985.28and NP_006738.2). The observed molecular weight corresponds to earlier findings inliterature with different antibodies (Kurachi et al, J Biol Chem.1997 Oct31,272(44): 28081-8, PMID: 9346962). An additional band of 55-60 kDa was consistentlyobserved, however this band was not blocked by the immunizing peptide and it istherefore a non-specific signal.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.

Target Details for SIPA1

Target: SIPA1 (Signal-Induced Proliferation-Associated 1 (SIPA1))

Alternative Name: SIPA1 / SPA1 (SIPA1 Products)

Synonyms: Spa1 antibody, SPA1 antibody, signal-induced proliferation-associated 1 antibody, Signal peptidase I (leader peptidase I) antibody, signal-induced proliferation associated gene 1 antibody, SIPA1 antibody, sipA1 antibody, Sipa1 antibody

Background: Synonyms: GTPase-activating protein Spa-1, Signal-induced proliferation-associated protein 1, Sipa-1, Spa-1, p130 SPA-1

Gene ID: 6494

UniProt: Q96FS4

Pathways: Response to Water Deprivation