AKAP3 antibody (C-Term)

Catalog No. ABIN374415

The Goat Polyclonal anti-AKAP3 antibody (ABIN374415) specifically detects AKAP3 in WB. The antibody is reactive with Human samples.

Quick Overview for AKAP3 antibody (C-Term) (ABIN374415)

Target: AKAP3 (A Kinase (PRKA) Anchor Protein 3 (AKAP3))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This AKAP3 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-AKAP3 Antibody

Binding Specificity: C-Term

Sequence: C-TPLQLLDWLM VNL

Specificity: This antibody detects AKAP3 at C-term.

Cross-Reactivity (Details): Species reactivity (tested):Human

Purification: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogen: Peptide from the C Terminus of the protein sequence according to NP_006413.2. Genename: AKAP3

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1: 32000. Western blot: 1-3 μg/mL. Approx 90 kDa band observed in Human Testis lysates (calculatedMW of 90 kDa according to NP_006413).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C.

Target Details for AKAP3

Target: AKAP3 (A Kinase (PRKA) Anchor Protein 3 (AKAP3))

Alternative Name: AKAP3

Background: A-kinase anchor proteins (AKAPs) direct the activity of protein kinase A (PKA) by tethering the enzyme near its physiologic substrates. AKAP3 belongs to the AKAP110 family. Expression is testis specific, and is only expressed in spermatids. It is thought to function as a regulator of both motility- and head-associated functions such as capacitation and the acrosome reaction in sperm.Synonyms: A-kinase anchor protein 110 kDa, A-kinase anchor protein 3, AKAP110, Cancer/testis antigen 82, Fibrous sheath protein of 95 kDa, Fibrousheathin I, Fibrousheathin-1, PRKA3, Protein kinase A-anchoring protein 3, SOB1, Sperm oocyte-binding protein

Gene ID: 10566

NCBI Accession: NP_006413

UniProt: O75969