RAN antibody (N-Term)

Catalog No. ABIN374447

The Goat Polyclonal anti-RAN antibody is suitable to detect RAN in samples from Human, Mouse, Rat, Dog and Cow. It has been validated for WB and EIA.

Quick Overview for RAN antibody (N-Term) (ABIN374447)

Target: RAN (RAN, Member RAS Oncogene Family (RAN))

Reactivity: Human, Mouse, Rat, Dog, Cow

Host: Goat

Clonality: Polyclonal

Conjugate: This RAN antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-RAN Antibody

Binding Specificity: N-Term

Sequence: C-AAQGEPQVQF KLV

Specificity: This antibody recognizes Ran (197-210).

Cross-Reactivity (Details): Species reactivity (expected):Rat, Canine (Dog), Bovine.
Species reactivity (tested):Human, Mouse.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide from the N-Terminus of the protein sequence according to NP_006316. Genename: RAN

Application Details

Application Notes: Peptide ELISA: 1/16,000. Western Blot: 1-3 μg/mL. Detects a band of Approx 25 kDa (Predicted Molecular Weight: 25 kDa). Positive Control: A431 and Jurkat whole cell lysate.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for RAN

Target: RAN (RAN, Member RAS Oncogene Family (RAN))

Alternative Name: RAN

Background: Ran (ras-related nuclear protein) is a small GTP binding protein belonging to the RAS superfamily that is essential for the translocation of RNA and proteins through the nuclear pore complex. The Ran protein is also involved in control of DNA synthesis and cell cycle progression. Nuclear localization of Ran requires the presence of regulator of chromosome condensation 1 (RCC1). Mutations in Ran disrupt DNA synthesis. Because of its many functions, it is likely that Ran interacts with several other proteins. Ran regulates formation and organization of the microtubule network independently of its role in the nucleus-cytosol exchange of macromolecules. Ran could be a key signaling molecule regulating microtubule polymerization during mitosis. RCC1 generates a high local concentration of Ran-GTP around chromatin which, in turn, induces the local nucleation of microtubules. Ran is an androgen receptor (AR) coactivator that binds differentially with different lengths of polyglutamine within the androgen receptor. Polyglutamine repeat expansion in the AR is linked to Kennedy's disease (X-linked spinal and bulbar muscular atrophy). Ran coactivation of the AR diminishes with polyglutamine expansion within the AR, and this weak coactivation may lead to partial androgen insensitivity during the development of Kennedy's disease.Synonyms: ARA24, Androgen receptor-associated protein 24, GTP-binding nuclear protein Ran, GTPase Ran, Ras-like protein TC4, Ras-related nuclear protein

Molecular Weight: 24.4 kDa (NP_006316.1)

Gene ID: 5901, 9606

UniProt: P62826

Pathways: Regulatory RNA Pathways, Intracellular Steroid Hormone Receptor Signaling Pathway, Protein targeting to Nucleus