Retinoid X Receptor alpha antibody (Internal Region)

Catalog No. ABIN374477

The Goat Polyclonal anti-Retinoid X Receptor alpha antibody is suitable to detect Retinoid X Receptor alpha in samples from Human, Mouse and Rat. It has been validated for WB and EIA.

Quick Overview for Retinoid X Receptor alpha antibody (Internal Region) (ABIN374477)

Target: Retinoid X Receptor alpha (RXRA) (Retinoid X Receptor, alpha (RXRA))

Reactivity: Human, Mouse, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This Retinoid X Receptor alpha antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-Retinoid X Receptor alpha Antibody

Binding Specificity: Internal Region

Sequence: CQVNSSLTSP TGRGSM

Specificity: This antibody is expected to recognise an epitope corresponding to aa 14-28 of Human RXR alpha protein and does not cross-react with either RXR beta or gamma.

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat.
Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide with sequence from the internal region of the protein sequence according to NP_002948.1. Genename: RXRA

Application Details

Application Notes: Peptide ELISA: 1/16000. Western blot: 0.5 - 3 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for Retinoid X Receptor alpha

Target: Retinoid X Receptor alpha (RXRA) (Retinoid X Receptor, alpha (RXRA))

Alternative Name: RXR-alpha / RXRA

Background: Synonyms: NR2B1, Nuclear receptor subfamily 2 group B member 1, Retinoic acid receptor RXR-alpha, Retinoid X receptor alpha

Gene ID: 6256, 9606

UniProt: P19793

Pathways: Nuclear Receptor Transcription Pathway, Retinoic Acid Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Regulation of Lipid Metabolism by PPARalpha, Hepatitis C