CCKBR antibody (Internal Region)

Catalog No. ABIN374567

The Goat Polyclonal anti-CCKBR antibody is suitable to detect CCKBR in samples from Human, Mouse, Rat and Cow. It has been validated for WB, IHC (p), IF and EIA.

Quick Overview for CCKBR antibody (Internal Region) (ABIN374567)

Target: CCKBR (Cholecystokinin B Receptor (CCKBR))

Reactivity: Human, Mouse, Rat, Cow

Host: Goat

Clonality: Polyclonal

Conjugate: This CCKBR antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Enzyme Immunoassay (EIA)

Product Details anti-CCKBR Antibody

Binding Specificity: Internal Region

Sequence: C-FDGDSDSDSQ SRVRNQ

Specificity: This antibody recognizes CCK-B Receptor.

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Bovine.
Species reactivity (tested):Human, Rat.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide

Immunogen: Peptide with sequence from the internal region of the protein sequence according to NP_795344.1. Genename: CCKBR

Application Details

Application Notes: Peptide ELISA: 1/32000. Western Blot: 0.1 - 0.3 μg/mL. Immunohistochemistry on paraffin sections: 3 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for CCKBR

Target: CCKBR (Cholecystokinin B Receptor (CCKBR))

Alternative Name: CCK-B Receptor

Background: CCKBR is a G protein coupled receptor for gastrin and cholecystokinin (CCK), regulatory peptides of the brain and gastrointestinal tract. This protein is a type B gastrin receptor, which has a high affinity for both sulfated and nonsulfated CCK analogs and is found principally in the central nervous system and the gastrointestinal tract. A misspliced transcript variant including an intron has been observed in cells from colorectal and pancreatic tumors. CCKBR is most abundant in brain and stomach but it can also be found in colon, pancreas, thyroid, and various cancers.Synonyms: CCK-BR, CCK2-R, CCKB Receptor, CCKBR, CCKRB, Cholecystokinin type B receptor, Cholecystokinin-2 receptor, Gastrin/cholecystokinin type B receptor

Gene ID: 887, 9606

UniProt: P32239

Pathways: Positive Regulation of Peptide Hormone Secretion, Feeding Behaviour