GAPDH antibody (Internal Region)

Catalog No. ABIN374640

This anti-GAPDH antibody is a Goat Polyclonal antibody detecting GAPDH in WB, IHC (fro) and EIA. Suitable for Human, Mouse, Rat and Dog. This Primary Antibody has been cited in 4+ publications.

Quick Overview for GAPDH antibody (Internal Region) (ABIN374640)

Target: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Reactivity: Human, Mouse, Rat, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This GAPDH antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Product Details anti-GAPDH Antibody

Binding Specificity: Internal Region

Sequence: C-GVNHEKYDNS LK

Specificity: This antibody is specific to GAPDH (Internal). GAPDH is constitutively expressed in almost all tissues at high levels. It is therefore a useful marker when a Loading/Positive Control is required in Western blotting.

Cross-Reactivity (Details): Species reactivity (expected):Canine (Dog).
Species reactivity (tested):Human, Mouse, Rat.

Purification: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide with sequence from the internal region of the protein sequence according to NP_002037.2. Genename: GAPDH

Application Details

Application Notes: Peptide ELISA: 1/4000 (Antibody detection limit dilution). Western Blot: 0.1-0.3 μg/mL. A approx. 36 kDa band is observed in Human Liver, Testes and Tonsillysates and in Mouse Spleen lysates.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-GAPDH antibody (ABIN374640)

Chakir, Daya, Aiba, Tunin, Dimaano, Abraham, Jaques-Robinson, Jacques, Lai, Pacak, Zhu, Xiao, Tomaselli, Kass: "Mechanisms of enhanced beta-adrenergic reserve from cardiac resynchronization therapy." in: Circulation, Vol. 119, Issue 9, pp. 1231-40, (2009) (PubMed).

Schillace, Miller, Pisenti, Grotzke, Swarbrick, Lewinsohn, Carr: "A-kinase anchoring in dendritic cells is required for antigen presentation." in: PLoS ONE, Vol. 4, Issue 3, pp. e4807, (2009) (PubMed).

Carr, Vugler, Lawrence, Chen, Ahmado, Chen, Semo, Gias, da Cruz, Moore, Walsh, Coffey: "Molecular characterization and functional analysis of phagocytosis by human embryonic stem cell-derived RPE cells using a novel human retinal assay." in: Molecular vision, Vol. 15, pp. 283-95, (2009) (PubMed).

Vugler, Carr, Lawrence, Chen, Burrell, Wright, Lundh, Semo, Ahmado, Gias, da Cruz, Moore, Andrews, Walsh, Coffey: "Elucidating the phenomenon of HESC-derived RPE: anatomy of cell genesis, expansion and retinal transplantation." in: Experimental neurology, Vol. 214, Issue 2, pp. 347-61, (2008) (PubMed).

Target Details for GAPDH

Target: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Alternative Name: GAPDH

Background: Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) is a metabolic enzyme originally thought to be only involved in Glycolysis. GAPDH is constitutively expressed at high levels in almost all tissues and serves therefore as a useful marker for the measurement of equal loading in western blotting experiments. Because of its ubiquitous expression, GAPDH is often seen as a housekeeping gene. However in the last two decades, every year a new function for this enzyme has been revealed. Besides its central role as a cytoplasmic enzyme in Glycolysis, recent evidence suggests that mammalian GAPDH is also involved in a great number of intracellular processes such as DNA replication and repair, nuclear membrane fusion, endocytosis, nuclear translocation in apoptosis, microtubule bundling, vesicular secretory transport, maintenance of telomere structure, phosphotransferase activity and nuclear RNA export. We now know that GAPDH is not only in the cytoplasm, but it shuttles between the cytoplasm and the nucleus and membranes. A lot of reports demonstrate the role of GAPDH in different pathologies such as progression in prostate cancer and ovarian cancer (mRNA stability), programmed neuronal cell death, and agerelated neuronal diseases. For example the sub-cellular distribution of GAPDH is abnormal in patients with Alzheimer's disease.Synonyms: CDABP0047, GAPD, Glyceraldehyde-3-Phosphate Dehydrogenase

Molecular Weight: 36.1kDa.

Gene ID: 2597

NCBI Accession: NP_002037

UniProt: P04406