DDX6 antibody (C-Term)

Catalog No. ABIN374654

The Goat Polyclonal anti-DDX6 antibody is suitable to detect DDX6 in samples from Human, Mouse, Rat and Dog. It has been validated for WB.

Quick Overview for DDX6 antibody (C-Term) (ABIN374654)

Target: DDX6 (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 6 (DDX6))

Reactivity: Human, Mouse, Rat, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This DDX6 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-DDX6 Antibody

Binding Specificity: C-Term

Specificity: This antibody reacts to DDX6.

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat, Canine.
Species reactivity (tested):Human.

Purification: Affinity chromatography

Immunogen: Peptide with sequence C-AEYHSEPVEDEKP, from the C Terminus of the protein sequence

Application Details

Application Notes: Peptide ELISA: 1/16000. Western Blot: 0.1 - 0.3 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for DDX6

Target: DDX6 (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 6 (DDX6))

Alternative Name: DDX6

Background: DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis and cellular growth and division. In the process of mRNA degradation, DDX6 may play a role in mRNA decapping. It forms a complex with DCP1A, DCP2, EDC3 and EDC4/HEDLS.Synonyms: ATP-dependent RNA helicase DDX6, ATP-dependent RNA helicase p54, DDX-6, DEAD box protein 6, HLR2, Oncogene RCK, RCK

Gene ID: 1656

NCBI Accession: NP_004388

UniProt: P26196

Pathways: Ribonucleoprotein Complex Subunit Organization