Patched 1 antibody (Internal Region)

Catalog No. ABIN374710

This Goat Polyclonal antibody specifically detects Patched 1 in WB, IF and EIA. It exhibits reactivity toward Human, Mouse, Dog, Cow and Pig.

Quick Overview for Patched 1 antibody (Internal Region) (ABIN374710)

Target: Patched 1 (PTCH1)

Reactivity: Human, Mouse, Dog, Cow, Pig

Host: Goat

Clonality: Polyclonal

Conjugate: This Patched 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA)

Product Details anti-Patched 1 Antibody

Binding Specificity: Internal Region

Specificity: This antibody detects PTCH.

Cross-Reactivity (Details): Species reactivity (expected):Canine (Dog), Pig, Cow.
Species reactivity (tested):Human, Mouse.

Purification: Affinity Chromatography

Immunogen: Peptide with sequence C-HPESRHHPPSNPRQQ from the internal region of the protein sequence Genename: PTCH

Application Details

Application Notes: ELISA: 1/32000. Western Blot: 1 - 3 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide and 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for Patched 1

Target: Patched 1 (PTCH1)

Alternative Name: PTCH1

Background: PTCH (Patched protein homolog 1) is a receptor for sonic hedgehog (SHH), indian hedgehog (IHH) and desert hedgehog (DHH). PTCH associates with the smoothened protein (SMO) to transduce the hedgehogs proteins signal.PTCH has a tumor suppressor function, as inactivation of this protein is probably a necessary, if not sufficient step for tumorigenesis. PTCH is expressed in the adult brain, lung, liver, heart, placenta, skeletal muscle, pancreas and kidney. It is also expressed in tumor cells but not in normal skin. During development PTCH is found in all major target tissues of sonic hedgehog, such as the ventral neural tube, somites, and tissues surrounding the zone of polarizing activity of the limb bud. Defects in PTCH are probably the cause of basal cell nevus syndrome also known as Gorlin syndrome or Gorlin-Goltz syndrome.Synonyms: PTC, PTC1, PTCH, Patched 1, Protein patched homolog 1

Gene ID: 5727

NCBI Accession: NP_000255

UniProt: Q13635

Pathways: Hedgehog Signaling, Carbohydrate Homeostasis, Tube Formation