Patched 1 antibody (C-Term)

Catalog No. ABIN2773841

Product Details anti-Patched 1 Antibody

Target: Patched 1 (PTCH1)

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat, Zebrafish (Danio rerio), Dog, Cow, Horse, Guinea Pig, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Patched 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Sequence: TILGVLNGLV LLPVLLSFFG PYPEVSPANG LNRLPTPSPE PPPSVVRFAM

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 93%, Rat: 100%, Zebrafish: 79%

Characteristics: This is a rabbit polyclonal antibody against PTCH1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human PTCH1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 345 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-Patched 1 antibody (ABIN2773841)

Meerang, Bérard, Felley-Bosco, Lauk, Vrugt, Boss, Kenkel, Broggini-Tenzer, Stahel, Arni, Weder, Opitz: "Antagonizing the Hedgehog Pathway with Vismodegib Impairs Malignant Pleural Mesothelioma Growth In Vivo by Affecting Stroma." in: Molecular cancer therapeutics, Vol. 15, Issue 5, pp. 1095-105, (2016) (PubMed).

Target Details for Patched 1

Target: Patched 1 (PTCH1)

Alternative Name: PTCH1 (PTCH1 Products)

Synonyms: BCNS antibody, HPE7 antibody, NBCCS antibody, PTC antibody, PTC1 antibody, PTCH antibody, PTCH11 antibody, lep antibody, ptc1 antibody, ptc2 antibody, Ptch antibody, Ptch2 antibody, Ptc-1 antibody, XPtc1 antibody, patched antibody, patched1 antibody, A230106A15Rik antibody, Ptc antibody, Ptc1 antibody, mes antibody, patched 1 antibody, patched 1 L homeolog antibody, Protein patched homolog 1 antibody, PTCH1 antibody, ptch1 antibody, Ptch1 antibody, ptch1.L antibody, ptc-1 antibody

Background: PTCH1 is a member of the patched gene family. The protein is the receptor for sonic hedgehog, a secreted molecule implicated in the formation of embryonic structures and in tumorigenesis. It functions as a tumor suppressor. Mutations of its gene have been associated with nevoid basal cell carcinoma syndrome, esophageal squamous cell carcinoma, trichoepitheliomas, transitional cell carcinomas of the bladder, as well as holoprosencephaly.
Alias Symbols: PTC, BCNS, HPE7, PTC1, PTCH, NBCCS, PTCH11
Protein Interaction Partner: ITCH, SMURF2, SMURF1, NEDD4L, WWP2, WWP1, UBC, NEDD4, FHL2, CASP9, SMO, CAV1, HIPK2, DHH, IHH, SHH, CCNB1, CDK1,
Protein Size: 345

Molecular Weight: 37 kDa

Gene ID: 5727

NCBI Accession: NM_000264

UniProt: Q6TKP8

Pathways: Hedgehog Signaling, Carbohydrate Homeostasis, Tube Formation