NOD2 antibody (Internal Region)

Catalog No. ABIN374736

The Goat Polyclonal anti-NOD2 antibody (ABIN374736) specifically detects NOD2 in WB. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for NOD2 antibody (Internal Region) (ABIN374736)

Target: NOD2 (Nucleotide-Binding Oligomerization Domain Containing 2 (NOD2))

Reactivity: Human, Mouse, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This NOD2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-NOD2 Antibody

Binding Specificity: Internal Region

Sequence: KFRFTDRERH CSPTD

Specificity: This antibody recognizes CARD15 / NOD2.

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat.
Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide

Immunogen: Peptide with sequence from the internal region of the protein sequence Genename: CARD15

Application Details

Application Notes: Peptide ELISA: 1/64000. Western Blot: 0.1 - 0.3 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % Sodium Azide, pH 7.3 with 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for NOD2

Target: NOD2 (Nucleotide-Binding Oligomerization Domain Containing 2 (NOD2))

Alternative Name: CARD15 / NOD2

Background: CARD15 (Nod2) is a member of the Nod1/Apaf-1 family of intracellular proteins that contain an N-terminal caspase recruitment domain (CARD), a centrally located nucleotide-binding domain (NBD), and a C-terminal regulatory domain. CARD15 induces NF-kappa-B via RICK (CARDIAK, RIP2) and IKK-gamma. It also confers responsiveness to intracellular bacterial lipopolysaccharides (LPS). Unlike Nod1, this homologue is primary expressed in monocytes. Defects in CARD15 are associated with Blau Syndrome (BS) and Crohn's Disease (CD), and are a cause of susceptibility to ulcerative colitis.Synonyms: Caspase recruitment domain-containing protein 15, IBD1, Inflammatory bowel disease protein 1, Nucleotide-binding oligomerization domain-containing protein 2

Gene ID: 64127, 9606

UniProt: Q9HC29

Pathways: Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Toll-Like Receptors Cascades, Inflammasome