PEBP1 antibody (Internal Region)

Catalog No. ABIN374748

The Goat Polyclonal anti-PEBP1 antibody (ABIN374748) specifically detects PEBP1 in WB and IHC (p). The antibody is reactive with Human, Mouse, Rat and Dog samples.

Quick Overview for PEBP1 antibody (Internal Region) (ABIN374748)

Target: PEBP1 (Phosphatidylethanolamine Binding Protein 1 (PEBP1))

Reactivity: Human, Mouse, Rat, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This PEBP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-PEBP1 Antibody

Binding Specificity: Internal Region

Specificity: This antibody reacts to PEBP1/RKIP.

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat and Canine.
Species reactivity (tested):Human.

Purification: Ammonium sulphate precipitation followed by antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-DPDAPSRKDPKYRE, from the internal region of the protein sequence according to NP_002558.1. Genename: PEBP1

Application Details

Application Notes: Peptide ELISA: Detection Limit: 1/128000. Western blot: 0.01-0.03 μg/mL. Approx 21 kDa band observed in Human Prostate lysates(calculated MW of 21.1 kDa according to NP_002558.1). Immunohistochemistry: 2-5 μg/mL. In paraffin embedded Adrenal Gland shows strongstinaing of secretory cells.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for PEBP1

Target: PEBP1 (Phosphatidylethanolamine Binding Protein 1 (PEBP1))

Alternative Name: PEBP1 / RKIP

Background: PBP, also known as RKIP (RAF kinase inhibitor protein), binds ATP, opioids and phosphatidylethanolamine. PBP is able to regulate Aurora kinase B expression and the spindle checkpoint through interaction with the RAF1/MEK/ERK cascade. PBP is able to bind to RAF1 and MEK and competitively disrupt the interaction between these proteins. PBP may be involved in the function of the presynaptic cholinergic neurons of the central nervous system. It increases the production of choline acetyltransferase but not acetylcholinesterase. PBP has been implicated as a suppressor of metastasis.Synonyms: HCNPpp, PBP, PEBP, PEBP-1, Phosphatidylethanolamine-binding protein 1, Prostatic-binding protein, Raf kinase inhibitor protein

Gene ID: 5037

NCBI Accession: NP_002558

UniProt: P30086

Pathways: Feeding Behaviour