PPID antibody (Internal Region)

Catalog No. ABIN374769

The Goat Polyclonal anti-PPID antibody (ABIN374769) specifically detects PPID in WB. The antibody is reactive with Human, Mouse, Rat and Dog samples.

Quick Overview for PPID antibody (Internal Region) (ABIN374769)

Target: PPID (Peptidylprolyl Isomerase D (PPID))

Reactivity: Human, Mouse, Rat, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This PPID antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-PPID Antibody

Binding Specificity: Internal Region

Specificity: This antibody reacts to PPID / CyP-40.

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat, Canine.
Species reactivity (tested):Human.

Purification: Affinity chromatography

Immunogen: Peptide with sequence C-DENFHYKHDREG, from the internal region of the protein sequence

Application Details

Application Notes: Peptide ELISA: 1/64000. Western Blot: 0.1 - 0.3 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for PPID

Target: PPID (Peptidylprolyl Isomerase D (PPID))

Alternative Name: Cyclophilin-40

Background: Cyclophilin 40 is a widely expressed member of the cyclophilin type PPIase family, PPIase D subfamily. It contains 1 PPIase cyclophilin type domain and 3 TPR repeats. It catalyzes the cis trans isomerization of proline imidic peptide bonds in oligopeptides. and has a role in accelerating the folding of proteins. It is less sensitive to inhibition by cyclosporin A than CYP18. Immunophilins are a family of soluble cytosolic receptors capable of binding to one of two major immunosuppressant agents: cyclosporin A (CsA) or FK506. Proteins that bind FK506 are termed FK506 Binding Proteins (FKBPs) and those that bind cyclosporin A are called cyclophilins (CyP). Both CyP:CsA and FKBP:FK506 complexes have been shown to inhibit calcineurin, a calcium and calmodulin dependent protein phosphatase which has been implicated as an important signaling enzyme in T-cell activation, providing a possible mechanism of immunosuppression by CsA and FK506. Immunophilins function as peptidyl prolyl cis-trans-isomerases (PPIase) whose activity is inhibited by their respective immunosuppressant compounds. As PPIase's, immunophilins accelerate folding of some proteins both in vivo and in vitro by catalyzing slow steps in the initial folding and rearrangement of proline containing proteins. CyP-40, a 40 kDa protein, shares significant homology with smaller CyPA (CyP-18) and FKBP59. CyP-40 exhibits the characteristic CsA binding and isomerase activity of CyP-18, though these activities appear to be less with CyP-40 than with Cyp-18. Like FKBP59, CyP-40 has been found in progesterone receptor complexes. CyP-40 is expressed at similar levels in many tissues.Synonyms: 40 kDa peptidyl-prolyl cis-trans isomerase, CYP-40, CYP40, CYPD, Cyclophilin D, Cyclophilin-related protein, PPID, PPIase D, Rotamase D

Gene ID: 5481, 9606

UniProt: Q08752

Pathways: Nuclear Hormone Receptor Binding