PTF1A antibody (Internal Region)

Catalog No. ABIN374789

The Goat Polyclonal anti-PTF1A antibody is suitable to detect PTF1A in samples from Human, Rat, Mouse, Zebrafish (Danio rerio) and Dog. It has been validated for WB and EIA.

Quick Overview for PTF1A antibody (Internal Region) (ABIN374789)

Target: PTF1A (Pancreas Specific Transcription Factor, 1a (PTF1A))

Reactivity: Human, Rat, Mouse, Zebrafish (Danio rerio), Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This PTF1A antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-PTF1A Antibody

Binding Specificity: Internal Region

Sequence: C-WTDEKQLKEQ N

Specificity: This antibody detects PTF1A / PFT1-P48.

Cross-Reactivity (Details): Species reactivity (expected):Rat, Canine (Dog), Zebrafish.
Species reactivity (tested):Human, Mouse.

Purification: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide

Immunogen: Peptide from the internal region of the protein sequence according to NP_835455.1. Genename: PTF1A

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1: 32000. Western blot: 0.5-1.5 μg/mL. Approx 48 kDa band observed in Human Brain (Cerebellum)lysates (calculated MW of 35.8 kDa according to NP_835455.1.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for PTF1A

Target: PTF1A (Pancreas Specific Transcription Factor, 1a (PTF1A))

Alternative Name: PTF1A

Background: PTF1A is a pancreas specific transcription factor. Mammalian studies have implicated important roles for the basic helix-loop-helix transcription factor PTF1A-p48 in the development of both exocrine and endocrine pancreas.Synonyms: BHLHA29, Class A basic helix-loop-helix protein 29, PTF1-p48, PTF1P48, Pancreas transcription factor 1 subunit alpha, Pancreas-specific transcription factor 1a, bHLH transcription factor p48

Gene ID: 256297

NCBI Accession: NP_835455

UniProt: Q7RTS3

Pathways: Retinoic Acid Receptor Signaling Pathway