Liver Arginase antibody (C-Term)

Catalog No. ABIN374812

This Goat Polyclonal antibody specifically detects Liver Arginase in WB. It exhibits reactivity toward Rat and Mouse.

Quick Overview for Liver Arginase antibody (C-Term) (ABIN374812)

Target: Liver Arginase (ARG1) (Arginase, Liver (ARG1))

Reactivity: Rat, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This Liver Arginase antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Liver Arginase Antibody

Binding Specificity: C-Term

Sequence: C-NHKPETDYLK PPK

Specificity: This antibody detects Arginase-1.

Cross-Reactivity (Details): Species reactivity (tested):Mouse, Rat.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide

Immunogen: Peptide from the C-Terminus of the protein sequence according to NP_058830.2 Genename: Arg1

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1: 32000. Western blot: 0.01-0.03 μg/mL. Approx 37 kDa band observed in Mouse Liver and Rat Liverlysates (calculated MW of 35.7 kDa according to NP_058830.1).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for Liver Arginase

Target: Liver Arginase (ARG1) (Arginase, Liver (ARG1))

Alternative Name: Arginase-1

Background: Arginase catalyzes the hydrolysis of Arginine to Ornithine and Urea. At least two isoforms of mammalian arginase exist (types I and II) which differ in their tissue distribution, subcellular localization, immunologic crossreactivity and physiologic function. The type I isoform, is a cytosolic enzyme and expressed predominantly in the liver as a component of the urea cycle. Inherited deficiency of this enzyme results in argininemia, an autosomal recessive disorder characterized by hyperammonemia.Synonyms: ARG1, Liver-type arginase, Type I arginase

Gene ID: 11846, 10090

UniProt: Q61176

Pathways: Cellular Response to Molecule of Bacterial Origin