ILF2 antibody (C-Term, Internal Region)

Catalog No. ABIN374823

This anti-ILF2 antibody is a Goat Polyclonal antibody detecting ILF2 in WB, IHC (p) and EIA. Suitable for Human, Mouse, Rat and Dog.

Quick Overview for ILF2 antibody (C-Term, Internal Region) (ABIN374823)

Target: ILF2 (Interleukin Enhancer Binding Factor 2, 45kDa (ILF2))

Reactivity: Human, Mouse, Rat, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This ILF2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-ILF2 Antibody

Binding Specificity: C-Term, Internal Region

Sequence: C-EKAYEKPPEK KEG

Specificity: This antibody recognizes ILF2/NF45.

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat and Canine (Dog).
Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Synthetic Peptide corresponding to the internal region (near the C Terminus) of Human ILF2 (NP_004506.2). Genename: ILF2

Application Details

Application Notes: Peptide ELISA: Detection Limit Dilution: 1/16000. Western Blot: 0.03-0.1 μg/mL. Detects a band of approx. 43 kDa in Human Tonsil and Thymuslysates (Predicted Molecular Weight: 43.3 kDa according to NP_004506.2).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for ILF2

Target: ILF2 (Interleukin Enhancer Binding Factor 2, 45kDa (ILF2))

Alternative Name: ILF2

Background: ILF2 appears to function predominantly as a heterodimeric complex with ILF3. This complex may regulate transcription of the IL2 gene during T-cell activation. ILF2 can also promote the formation of stable DNA-dependent protein kinase holoenzyme complexes on DNA.Synonyms: ILF-2, Interleukin enhancer-binding factor 2, NF-45, NF45, Nuclear factor of activated T-cells 45 kDa

Molecular Weight: 43.1kDa (NP_004506.2).

Gene ID: 3608

NCBI Accession: NP_004506

UniProt: Q12905