TBC1D4 antibody (Internal Region)

Catalog No. ABIN374867

The Goat Polyclonal anti-TBC1D4 antibody is suitable to detect TBC1D4 in samples from Human and Dog. It has been validated for WB and IHC (p).

Quick Overview for TBC1D4 antibody (Internal Region) (ABIN374867)

Target: TBC1D4 (TBC1 Domain Family, Member 4 (TBC1D4))

Reactivity: Human, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This TBC1D4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-TBC1D4 Antibody

Binding Specificity: Internal Region

Sequence: C-DDPEKIEERK KSK

Specificity: This antibody detects internal TBC1D4 / AS160.

Cross-Reactivity (Details): Species reactivity (expected):DogSpecies reactivity (tested):Human

Purification: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogen: Peptide from the internal region of the protein sequence according to NP_055647.2 Genename: TBC1D4

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1: 128000. Western Blot: Approx 150 kDa band observed in lysates of cell lines Daudi and MOLT4(calculated MW of 140 kDa according to NP_055647.2). Recommended concentration: 0.1-0.3 μg/mL. Immunohistochemistry on paraffin sections: 3-5 μg/mL. In Human Skeletal Muscle shows striated pattern staining of majority of muscular fibres.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer.

Target Details for TBC1D4

Target: TBC1D4 (TBC1 Domain Family, Member 4 (TBC1D4))

Alternative Name: TBC1D4 / AS160

Background: TBC1D4 is a Rab GTPase activating protein (GAP) for Rab family protein(s) (referenced from swissprot).Synonyms: Akt substrate of 160 kDa, KIAA0603, TBC1 domain family member 4

Gene ID: 9882, 9606

UniProt: O60343