SLC47A1 antibody (Internal Region)

Catalog No. ABIN375104

The Goat Polyclonal anti-SLC47A1 antibody is suitable to detect SLC47A1 in samples from Human. It has been validated for WB.

Quick Overview for SLC47A1 antibody (Internal Region) (ABIN375104)

Target: SLC47A1 (Solute Carrier Family 47, Member 1 (SLC47A1))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This SLC47A1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-SLC47A1 Antibody

Binding Specificity: Internal Region

Sequence: C-PEHPQDGAKL SRKQ

Specificity: This antibody is specific to SLC47A1/MATE-1 (Internal).

Purification: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography.

Immunogen: Peptide with sequence from the internal region (near C-Terminus) of the protein sequence according to NP_060712.2.

Application Details

Application Notes: Peptide ELISA: Antibody detection limit dilution 1/64,000. Western Blot: (0.1-0.3 μg/mL)A approx. 60 kDa band is observed in Human Kidney lysates. An additional band of unknownidentity was also consistently observed at 20 kDa. This band was successfully blocked byincubation with the immunizing peptide.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for SLC47A1

Target: SLC47A1 (Solute Carrier Family 47, Member 1 (SLC47A1))

Alternative Name: SLC47A1

Background: Transporters are expressed in a wide variety of tissues where they perform the critical function of enabling anionic and cationic chemicals of exogenous and endogenous origin to cross otherwise impermeable cell membranes. The multidrug and toxin extrusion (MATE) transporters mediate cellular efflux of a variety of organic cations, including many drugs, and are involved in excretion of toxic electrolytes through urine and bile. Members of the MATE family share homology with the bacterial MATE protein family, which is responsible for drug resistance. MATE1 (multidrug and toxin extrusion protein 1), also known as SLC47A1, is a 570 amino acid multi-pass membrane protein that predominantly localizes to the plasma membrane but is also found in intracellular organelles. Expressed in adrenal gland, and to a lower extent in liver, skeletal muscle and kidney, MATE1 is responsible for the secretion of cationic drugs across the brush border membranes. MATE1 exists as three isoforms, which are produced by alternative splicing events.Synonyms: MATE-1, MATE1, Multidrug and toxin extrusion protein 1, Solute carrier family 47 member 1

Gene ID: 55244, 9606

UniProt: Q96FL8