GPI antibody (pSer185)

Catalog No. ABIN375349

This Rabbit Polyclonal antibody specifically detects GPI in WB, ELISA and IHC. It exhibits reactivity toward Human.

Quick Overview for GPI antibody (pSer185) (ABIN375349)

Target: GPI (Glucose-6-Phosphate Isomerase (GPI))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GPI antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-GPI Antibody

Binding Specificity: pSer185

Specificity: Reacts with human 65 kDa GPI protein with a phosphoserine at position 185

Cross-Reactivity (Details): Do not cross react with non-phospho GPI protein.

Purification: Antiserum

Immunogen: Synthetic peptide derived from human GPI containing a phosphoserine at position 185

Isotype: IgG

Application Details

Application Notes:

Working dilution: Optimal dilution should be determined by the end user.
The following are guidelines only :
- IHC : 1/200 to 1/1 000 - WB : 1/500 to 1/5 000

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Must be reconstituted in distilled water.

Storage: 4 °C/-20 °C

Storage Comment: Lyophilized powder stable for a minimum of 2 years at -20°C. Store reconstituted antibodies at +4°C. For extended periods store in aliquots at -20°C. Antibodies are guaranteed for 6 month from date of receipt.

Expiry Date: 24 months

Target Details for GPI

Target: GPI (Glucose-6-Phosphate Isomerase (GPI))

Background: Besides it's role as a glycolytic enzyme, mammalian GPI can function as a tumor-secreted cytokine and an angiogenic factor (AMF) that stimulates endothelial cell motility. GPI is also a neurotrophic factor (Neuroleukin) for spinal and sensory neurons. Phosphorylation at Ser-185 by CK2 has been shown to decrease enzymatic activity and may contribute to secretion by a non-classical secretory pathway.

UniProt: P06744