CD25 antibody (Biotin)

Catalog No. ABIN375432

This anti-CD25 antibody is a Rat Monoclonal antibody detecting CD25 in FACS. Suitable for Mouse.

Quick Overview for CD25 antibody (Biotin) (ABIN375432)

Target: CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This CD25 antibody is conjugated to Biotin

Application: Flow Cytometry (FACS)

Clone: 3C7

Product Details anti-CD25 Antibody (Biotin)

Specificity: Mouse CD25, Mr 55 kDa

Characteristics: Rat Anti-Mouse CD25-BIOT

Purification: Purified

Immunogen: IL-2 dependent BALB/c helper T cell clone HT2

Isotype: IgG2b

Application Details

Application Notes:

• Applications: FC - Quality tested , IHC-FS - Reported in literature , IP - Reported in literature , Block - Reported in literature , Neut - Reported in literature
• Working Dilutions: Flow Cytometry FITC and BIOT conjugates 3 g/106 cells PE, APC, SPRD, PE/CY7, and AF700 conjugates 0.3 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L

Comment:

Inhibition of IL-2 or mitogen-induced T cell proliferation, In vitro blocking of IL-2 binding

Sample Volume: 1 mL

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: 0.5 mg in 1.0 mL of PBS/Sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect conjugated products from light.
Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C

Storage Comment: Store at 2-8°C

Target Details for CD25

Target: CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))

Alternative Name: CD25

Background: The IL-2 receptor (IL-2R) exists in three alternative forms made up from the individual components of CD25, CD122, and CD132. CD25 represents the low affinity α chain of the IL-2R, a type I transmembrane glycoprotein containing two CCP domains. It is rich in O-linked carbohydrates and has a short cytoplasmic tail. CD25 is expressed on activated T cells, B cells, NK cells and monocytes of all mouse strains tested. Expression of CD25 on activated T lymphocytes is transitory and endogenously regulated. CD25 is expressed on precursor B cells in bone marrow. Its expression is initiated by functional rearrangement and expression of IgM heavy chain genes and is down-regulated when immature B cells mature and express IgD. It is expressed at a higher level on CD4 + CD8 + thymocytes. It is also expressed on cultured epidermal Langerhans cells. The biochemical features of murine CD25 have been characterized in detail.

Pathways: JAK-STAT Signaling, Growth Factor Binding, Activated T Cell Proliferation