ZAP70 antibody (AA 280-309) (Biotin)

Catalog No. ABIN375443

The Mouse Monoclonal anti-ZAP70 antibody has been validated for FACS and ELISA. It is suitable to detect ZAP70 in samples from Human.

Quick Overview for ZAP70 antibody (AA 280-309) (Biotin) (ABIN375443)

Target: ZAP70 (zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ZAP70 antibody is conjugated to Biotin

Application: Flow Cytometry (FACS), ELISA

Clone: SBZAP

Product Details anti-ZAP70 Antibody (Biotin)

Binding Specificity: AA 280-309

Specificity: Human ZAP-70

Characteristics: Mouse Anti-Human ZAP-70-BIOT

Purification: Purified

Immunogen: Human ZAP-70 (a.a. 280-309)

Isotype: IgG1

Application Details

Application Notes:

• Applications: FC - Quality tested , ELISA - Quality tested
• Working Dilutions: Flow Cytometry Purified (UNLB) antibody ≤ 1 g/106 cells FITC and BIOT conjugates ≤ 1 g/106 cells PE conjugate ≤ 0.2 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L ELISA AP conjugate 1:500 - 1:1,000 HRP conjugate 1:1,000 - 1:2,000

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: 0.025 mg in 0.25 mL or 0.1 mg in 1.0 mL of PBS/Sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C

Storage Comment: Store at 2-8°C

Target Details for ZAP70

Target: ZAP70 (zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70))

Alternative Name: ZAP-70

Background: ZAP 70, also known as TCR associated protein, is a protein tyrosine kinase (PTK) with the primary role of catalyzing the phosphorylation of tyrosine residues in cellular proteins, and thus, contributes significantly to the initiation of cell signaling. ZAP 70 is expressed on T cells and NK cells. The monoclonal antibodies SB70 and SBZAP were generated against a KLH-peptide sequence corresponding to the human ZAP 70 AA 280-309.

Pathways: TCR Signaling, Ubiquitin Proteasome Pathway