ICAM1 antibody

Catalog No. ABIN375585

Product Details anti-ICAM1 Antibody

Target: ICAM1 (Intercellular Adhesion Molecule 1 (ICAM1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ICAM1 antibody is un-conjugated

Application: Flow Cytometry (FACS)

Specificity: Human/Porcine CD54, Mr 90 kDa

Characteristics: Mouse Anti-Human CD54-UNLB

Purification: Purified

Immunogen: Rheumatoid synovial cells and human monocytes

Clone: 15-2

Isotype: IgG1

Application Details

Application Notes:

• Applications: FC - Quality tested , IHC-FS - Reported in literature , ICC - Reported in literature , IP - Reported in literature , WB - Reported in literature , Block - Reported in literature , Neut - Reported in literature , ELISA - Reported in literature
• Working Dilutions: Flow Cytometry Purified (UNLB) antibody 1 g/106 cells FITC, BIOT, PE, and APC conjugates 10 L/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L

Comment:

Blocking Studies

Sample Volume: 1 mL

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: 0.1 mg of purified immunoglobulin in 1.0 mL of borate buffered saline,  pH 8.2. No preservatives or amine-containing buffer salts added

Preservative: Without preservative

Handling Advice: Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C

Storage Comment: Store at 2-8°C

Target Details for ICAM1

Target: ICAM1 (Intercellular Adhesion Molecule 1 (ICAM1))

Alternative Name: CD54 (ICAM1 Products)

Target Type: Viral Protein

Background: CD54, also known as intercellular cell adhesion molecule-1 (ICAM-1), is a 90-110 kDa type I transmembrane glycoprotein and a member of the immunoglobulin superfamily. It exhibits a wide tissue distribution, being expressed on both hematopoietic and non-hematopoietic cells. ICAM-1 expression on leukocytes is low but is rapidly upregulated upon activation. Expression on endothelium and other non-hematopoietic cells is strongly upregulated by inflammatory mediators. The ligands of ICAM-1 are CD11a/LFA-1 α and CD11b/Mac-1 α. Endothelial CD54 contributes to the extravasation of leukocytes from blood vessels, particularly in areas of inflammation. CD54 on antigen-presenting cells (APC) contributes to antigen-specific T cell activation, presumably by enhancing interactions between T cells and APC.

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Regulation of Leukocyte Mediated Immunity, Thromboxane A2 Receptor Signaling