Goat anti-Rat IgG (Heavy & Light Chain) Antibody (Biotin) - Preadsorbed

Catalog No. ABIN375969

Quick Overview for Goat anti-Rat IgG (Heavy & Light Chain) Antibody (Biotin) - Preadsorbed (ABIN375969)

Target: IgG

Clonality: Polyclonal

Conjugate: Biotin

Application: ELISA, Western Blotting (WB), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Separation (Sep)

Isotype: IgG

Product Details

Binding Specificity: Heavy & Light Chain

Reactivity: Rat

Host: Goat

Purpose: Goat Anti-Rat IgG(H+L), Mouse ads-BIOT

Specificity: Reacts with the heavy and light chains of rat IgG, and with the light chains of rat IgM and IgA as demonstrated by ELISA and/or flow cytometry.

Characteristics: Goat Anti-Rat IgG-Biotin antibody with minimal reactivity to mouse proteins for use in ELISA, flow cytometry, immunohistochemistry / immunocytochemistry, western blotting, and separation assays.

Purification:

Purified by affinity chromatography on pooled rat IgG covalently linked to agarose, cross-adsorbed against mouse immunoglobulins and pooled sera for minimal reactivity

Preadsorption: Mouse pre-adsorbed

Immunogen: Rat IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Pooled antisera from goats hyperimmunized with rat IgG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Phosphate buffered saline containing < 0.1 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: 2-8°C

References

van den Borne, Rygiel, Hoogendoorn, Westerlaken, Boon, Quax, Pasterkamp, Hoefer, Meyaard: "The CD200-CD200 receptor inhibitory axis controls arteriogenesis and local T lymphocyte influx." in: PLoS ONE, Vol. 9, Issue 6, pp. e98820, (2014) (PubMed).

Target Details

Target: IgG

Target Type: Antibody

Background: Optional[Description]: Goat Anti-Rat IgG(H+L), Mouse ads-BIOT