Goat anti-Human IgG Antibody (PE) - Preadsorbed

Catalog No. ABIN376512

Quick Overview for Goat anti-Human IgG Antibody (PE) - Preadsorbed (ABIN376512)

Target: IgG

Fragment: F(ab')2 fragment

Clonality: Polyclonal

Conjugate: PE

Application: Flow Cytometry (FACS), FLISA

Isotype: IgG

Product Details

Reactivity: Human

Host: Goat

Purpose: Goat F(ab')2 Anti-Human IgG, Mouse ads-PE

Specificity: Reacts with the heavy chain of human IgG

Characteristics: Goat F(ab')2 Anti-Human IgG-PE antibody with minimal reactivity to mouse proteins for use in flow cytometry assays.

Purification:

Purified by affinity chromatography on human IgG covalently linked to agarose, cross-adsorbed against human IgM and IgA / mouse immunoglobulins and pooled sera for minimal reactivity

Preadsorption: Mouse pre-adsorbed

Immunogen: Human IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Pepsin digest of goat anti-human IgG (SouthernBiotech Cat. No. 2044) - Pooled antisera from goats hyperimmunized with human IgG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: Phosphate buffered saline containing < 0.1 % sodium azide and a stabilizer

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: 2-8°C

References

Brannan, He, Howell, Prugar, Zhu, Vu, Shulenin, Kailasan, Raina, Wong, Rahim, Banadyga, Tierney, Zhao, Li, Holtsberg, Dye, Qiu, Aman: "Post-exposure immunotherapy for two ebolaviruses and Marburg virus in nonhuman primates." in: Nature communications, Vol. 10, Issue 1, pp. 105, (2019) (PubMed).

Gallery, Zhang, Bradley, Brauer, Cvet, Estevam, Danaee, Greenfield, Li, Manfredi, Loke, Rabino, Stringer, Williamson, Wyant, Yang, Zhu, Abu-Yousif, Veiby: "A monomethyl auristatin E-conjugated antibody to guanylyl cyclase C is cytotoxic to target-expressing cells in vitro and in vivo." in: PLoS ONE, Vol. 13, Issue 1, pp. e0191046, (2018) (PubMed).

Kristian, Ota, Bubeck, Cho, Groff, Kubota, Destito, Martin, Laudenslager, Koriazova, Tahara, Kanda: "Generation and Improvement of Effector Function of a Novel Broadly Reactive and Protective Monoclonal Antibody against Pneumococcal Surface Protein A of Streptococcus pneumoniae." in: PLoS ONE, Vol. 11, Issue 5, pp. e0154616, (2017) (PubMed).

Schubert, Bode, Kenefeck, Hou, Wing, Kennedy, Bulashevska, Petersen, Schäffer, Grüning, Unger, Frede, Baumann, Witte, Schmidt, Dueckers, Niehues, Seneviratne, Kanariou, Speckmann, Ehl, Rensing-Ehl et al.: "Autosomal dominant immune dysregulation syndrome in humans with CTLA4 mutations. ..." in: Nature medicine, Vol. 20, Issue 12, pp. 1410-1416, (2015) (PubMed).

Target Details

Target: IgG

Target Type: Antibody

Background: Optional[Description]: Goat F(ab')2 Anti-Human IgG, Mouse ads-PE