Mouse anti-Human IgG4 (Fc Region) Antibody (PE)

Catalog No. ABIN376574

Quick Overview for Mouse anti-Human IgG4 (Fc Region) Antibody (PE) (ABIN376574)

Target: IgG4

Clonality: Monoclonal

Conjugate: PE

Application: ELISA

Clone: HP6025

Isotype: IgG1

Product Details

Binding Specificity: Fc Region

Reactivity: Human

Host: Mouse

Specificity: Human IgG4 Fc, Mr 146 kDa

Characteristics: Mouse Anti-Human IgG4 Fc-PE

Purification: Purified

Immunogen: Human IgG4 myeloma protein

Application Details

Application Notes:

• Applications: ELISA - Quality tested , FLISA - Quality tested FC - Reported in literature , IHC-FS - Reported in literature , IHC-PS - Reported in literature , ICC - Reported in literature , WB - Reported in literature , Microarray - Reported in literature , Multiplex - Reported in literature , Purification - Reported in literature
• Working Dilutions: ELISA AP conjugate 1:1,000 - 1:2,000 HRP conjugate 1:4,000 - 1:8,000 BIOT conjugate 1:5,000 - 1:10,000 FLISA FITC, AF488, and AF555 conjugates 1:200 - 1:400 PE and AF647 conjugates ≤ 1 g/mL

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: 0.1 mg in 1.0 mL of PBS/Sodium azide and a stabilizing agent

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze!
Protect conjugated products from light.
Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C

Storage Comment: Store at 2-8°C

References

Violari, Cotton, Kuhn, Schramm, Paximadis, Loubser, Shalekoff, Da Costa Dias, Otwombe, Liberty, McIntyre, Babiker, Gibb, Tiemessen: "A child with perinatal HIV infection and long-term sustained virological control following antiretroviral treatment cessation." in: Nature communications, Vol. 10, Issue 1, pp. 412, (2019) (PubMed).

Howie, Delaney, Wang, Er, Vidarsson, Stegmann, Kapp, Lebedev, Wu, AuBuchon, Zimring: "Serological blind spots for variants of human IgG3 and IgG4 by a commonly used anti-immunoglobulin reagent." in: Transfusion, Vol. 56, Issue 12, pp. 2953-2962, (2017) (PubMed).

Song, Franco, Kao, Yu, Huang, Ho: "Epitope Mapping of Ibalizumab, a Humanized Anti-CD4 Monoclonal Antibody with Anti-HIV-1 Activity in Infected Patients." in: Journal of virology, (2010) (PubMed).

Target Details

Target: IgG4

Target Type: Antibody