CD69 antibody (CD69) (SPRD)

Details for Product anti-CD69 Antibody No. ABIN376714
Target
Reactivity
Mouse
107
71
29
7
7
7
5
4
4
2
1
Host
Hamster
58
55
35
9
Clonality (Clone)
Monoclonal ()
Conjugate
This CD69 antibody is conjugated to SPRD
22
16
12
11
4
3
3
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS)
114
44
32
31
27
21
13
9
8
8
5
4
3
3
1
Options
Brand Spectral Red ™
Immunogen Mouse dendritic epidermal cell line Y245
Clone H1-2F3
Isotype IgG
Specificity Mouse CD69, Mr 85 kDa (unreduced)
Characteristics Hamster Anti-Mouse CD69-SPRD
Purification Purified
Target
Alternative Name CD69 (CD69 Antibody Abstract)
Background CD69, also known as very early activation (VEA) antigen, is a disulfide-linked transmembrane homodimer whose differentially glycosylated subunits range from 35-39 kDa. It is a C-type lectin, most closely related to the NKR-P1 and Ly-49 NK cell-activation molecules. CD69 is widely expressed on hematopoietic cells, including lymphocytes, neutrophils and eosinophils. Although not detectable on resting lymphocytes, its expression is rapidly (within 2 hours) upregulated upon activation of T, B and NK cells, and neutrophils. Constitutive expression of CD69 on subsets of thymocytes suggests that it may be involved in regulation of developmental events in addition to its role in activation of a variety of hematopoietic cells.
Application Notes
  • Applications: FC - Quality tested , IHC-FS - Reported in literature , IP - Reported in literature , Costim - Reported in literature , Activ - Reported in literature
  • Working Dilutions: Flow Cytometry FITC, BIOT, and AF488 conjugates 1 g/106 cells PE, APC, SPRD, PE/CY7, AF647, and PACBLU conjugates 0.2 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L
Comment

In vitro T cell and NK-cell activation

Sample Volume 1 mL
Restrictions For Research Use only
Concentration 0.1 mg/mL
Buffer 0.1 mg in 1.0 mL of PBS/Sodium azide and a stabilizing agent
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze!
Protect conjugated products from light.

Each reagent is stable for the period shown on the bottle label if stored as directed.
Storage 4 °C
Storage Comment Store at 2-8°C
Images
Image no. 1 for anti-CD69 (CD69) antibody (SPRD) (ABIN376714) PMA and ionomycin stimulated BALB/c mouse splenocytes were stained with Hamster Anti-...