Biotin antibody

Catalog No. ABIN376975

This Mouse Monoclonal antibody specifically detects Biotin in ELISA and FACS. It exhibits reactivity toward .

Quick Overview for Biotin antibody (ABIN376975)

Target: Biotin

Reactivity: Please inquire

Host: Mouse

Clonality: Monoclonal

Conjugate: This Biotin antibody is un-conjugated

Application: ELISA, Flow Cytometry (FACS)

Clone: SB58c

Product Details anti-Biotin Antibody

Specificity: Biotin

Characteristics: Mouse Anti-BIOT-UNLB

Purification: Purified

Immunogen: Biotin-KLH

Isotype: IgG1

Application Details

Application Notes:

• Applications: ELISA - Quality tested FLISA - Quality tested FC - Quality tested WB - Reported in literature , PLA - Reported in literature
• Working Dilutions: Flow Cytometry FITC conjugate 1 g/106 cells PE conjugate 0.1 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L ELISA Purified (UNLB) antibody 1 g/mL HRP conjugate 1:4,000 - 1:8,000 AP conjugate 1:1,000 - 1:2,000 FLISA FITC conjugate 1:200 - 1:400 PE conjugate 1 g/mL

Sample Volume: 1 mL

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: 0.5 mg purified immunoglobulin in 1.0 mL of borate buffered saline,  pH 8.2. No preservatives or amine-containing buffer salts added

Preservative: Without preservative

Handling Advice: Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C

Storage Comment: Store at 2-8°C

Target Details for Biotin

Target: Biotin

Target Type: Chemical

Background: Anti-Biotin can be used for indirect immuno-staining when working with a biotinylated primary antibody. It can be used for simultaneous staining of several biotinylated antibodies as one of the parameters in certain assay platforms (such as Luminex). It is an alternative technology to magnify the signal and to reduce background caused by avidin or its derivatives.