Actin antibody (Asp244)

Catalog No. ABIN377124

The Rabbit Polyclonal anti-Actin antibody (ABIN377124) specifically detects Actin in WB and IHC. The antibody is reactive with Human, Rat, Mouse, Monkey and Drosophila melanogaster samples.

Quick Overview for Actin antibody (Asp244) (377124)

Target: Actin

Reactivity: Human, Rat, Mouse, Monkey, Drosophila melanogaster

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Actin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-Actin Antibody

Binding Specificity: Asp244

Specificity: Recognizes endogenous levels of total actin (all isoforms). Detects the 30 kD actin fragment cleaved at glutamate 107. Species cross-reactivity: human mouse rat, monkey and Drosophila.

Purification: Protein A purified

Immunogen: Synthetic peptide (KLH- coupled) corresponding to residues surrounding Asp244 of human b-actin.

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC (1:25), WB (1:1000)

Usage: Suitable for use in Western Blot and Immunohistochemistry. Western Blot: 1:1000. Immunohistochemistry:1:25.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 10 mM sodium HEPES,  pH 7.5, 150 mM sodium chloride, 100 μg/mL BSA, 50 % glycerol, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for Actin

Target: Actin