Actin antibody (Asp244)

Catalog No. ABIN377124

This anti-Actin antibody is a Rabbit Polyclonal antibody detecting Actin in WB and IHC. Suitable for Human, Rat, Mouse, Monkey and Drosophila melanogaster.

Quick Overview for Actin antibody (Asp244) (ABIN377124)

Target: Actin

Reactivity: Human, Rat, Mouse, Monkey, Drosophila melanogaster

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Actin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-Actin Antibody

Binding Specificity: Asp244

Specificity: Recognizes endogenous levels of total actin (all isoforms). Detects the 30 kD actin fragment cleaved at glutamate 107. Species cross-reactivity: human mouse rat, monkey and Drosophila.

Purification: Protein A purified

Immunogen: Synthetic peptide (KLH- coupled) corresponding to residues surrounding Asp244 of human b-actin.

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC (1:25), WB (1:1000)

Usage: Suitable for use in Western Blot and Immunohistochemistry. Western Blot: 1:1000. Immunohistochemistry:1:25.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 10 mM sodium HEPES,  pH 7.5, 150 mM sodium chloride, 100 μg/mL BSA, 50 % glycerol, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for Actin

Target: Actin