S100A8 antibody (Biotin)

Catalog No. ABIN379949

The Mouse Monoclonal anti-S100A8 antibody has been validated for IHC, ELISA and IHC (p). It is suitable to detect S100A8 in samples from Human.

Quick Overview for S100A8 antibody (Biotin) (ABIN379949)

Target: S100A8 (S100 Calcium Binding Protein A8 (S100A8))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This S100A8 antibody is conjugated to Biotin

Application: Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 8-5C2

Product Details anti-S100A8 Antibody (Biotin)

Specificity: The antigen is MRP8, the epitope is suspected in the central portion of the peptide. Antigen distribution: Isolated cells: The antigen is found in granulocytes and monocytes but not in other blood cells. In cultured monocytes, maximum MRP8 expression is found after 3 - 4 days. Myeloid leukaemia stains positively. Tissue sections: MRP8 is found in a distinct subpopulation of inflammatory perivascular infiltrates of the myelo-monocytic lineage. Macrophages synthesise MRP8 increasingly during the late stages of inflammation. A low MRP8 (and high MRP- 14) expression by macrophages was also reported in granulomatous diseases such as tuberculosis and sarcoidis. In non-granulomatous chronic inflammatory diseases such as chronic rheumatoid arthritis or chronic rejection after allograft transplantation, MRP8 and MRP14 positive cells consist of different subpopulations. Specificity: Human: MRP8 in stimulated monocytes and macrophages in late phase or chronic inflammation. Other: The antibody reacts with bovine spleen but does not react with rat tissues. It shows a diffuse reaction with swine spleen.

Purification: Affinity purified

Immunogen: Cultured human monocytes

Application Details

Application Notes: Approved: ELISA, IHC, IHC-P

Usage: The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.5 mL distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS  pH 7.2, 10 mg/mL BSA, 0.01 % thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 4°C.

Target Details for S100A8

Target: S100A8 (S100 Calcium Binding Protein A8 (S100A8))

Alternative Name: S100A8 / MRP8

Background: Name/Gene ID: S100A8

Synonyms: S100A8, 60B8AG, CFAG, CAGA, Calgranulin A, Calgranulin-A, Calprotectin L1L subunit, Cystic fibrosis antigen, CGLA, L1Ag, NIF, p8, Protein S100-A8, MRP8, Urinary stone protein band A, CP-10, MA387, MRP-8

Gene ID: 6279

UniProt: P05109

Pathways: Transition Metal Ion Homeostasis, Positive Regulation of Endopeptidase Activity, S100 Proteins