His Tag antibody (Biotin)

Catalog No. ABIN387702

This anti-His Tag antibody is a Mouse Monoclonal antibody detecting His Tag in WB and ELISA. Suitable for . This Primary Antibody has been cited in 2+ publications.

Quick Overview for His Tag antibody (Biotin) (ABIN387702)

Target: His Tag

Reactivity: Please inquire

Host: Mouse

Clonality: Monoclonal

Conjugate: This His Tag antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA

Clone: 6G2A9

Product Details anti-His Tag Antibody (Biotin)

Brand: THE™

Sequence: HHHHHH

Specificity: THE^TM His Tag Antibody [Biotin], mAb, Mouse specificly recognizes C-terminal, N-terminal, and internal His tagged fusion proteins.

Characteristics: The antibody is suitable for detecting fusion proteins that contain a His-tag and it is supplied as a 100 µL aliquot at a concentration of 1 mg/mL.

Purification: Protein A affinity column

Immunogen: A synthetic peptide (HHHHHH) coupled - KLH

Isotype: IgG

Application Details

Application Notes: Working concentrations for specific applications should be determined by the investigator. Appropriate concentrations will be affected by several factors, including secondary antibody affinity, antigen concentration, sensitivity of detection method, temperature, and length of incubations, etc. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.02-0.2 µg/mL
Western blot: 0.1-1.0 µg/mL
Other applications: user-optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: 0.5 mg/mL

Buffer: PBS, pH 7.4, containing 1 % BSA and 0.01 % Sodium azide

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freezing and thawing cycles.

Storage: 4 °C/-20 °C

Storage Comment: The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

References for anti-His Tag antibody (Biotin) (ABIN387702)

Wegman, Geuze, van der Helm, Cumhur Öner, Dhert, Alblas: "Gene delivery of bone morphogenetic protein-2 plasmid DNA promotes bone formation in a large animal model." in: Journal of tissue engineering and regenerative medicine, Vol. 8, Issue 10, pp. 763-70, (2014) (PubMed).

Ota, Doyle-Cooper, Cooper, Huber, Falkowska, Doores, Hangartner, Le, Sok, Jardine, Lifson, Wu, Mascola, Poignard, Binley, Chakrabarti, Schief, Wyatt, Burton, Nemazee: "Anti-HIV B Cell lines as candidate vaccine biosensors." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 189, Issue 10, pp. 4816-24, (2012) (PubMed).

Target Details for His Tag

Target: His Tag

Target Type: Tag