HA-Tag antibody

Catalog No. ABIN387711

Cited in 7+ publications. The Mouse Monoclonal anti-HA-Tag antibody (Clone 5E11D8) (ABIN387711) specifically detects HA-Tag in WB and ELISA.

Quick Overview for HA-Tag antibody (ABIN387711)

Target: HA-Tag

Host: Mouse

Clonality: Monoclonal

Conjugate: This HA-Tag antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Clone: 5E11D8

Product Details anti-HA-Tag Antibody

Sequence: YPYDVPDYA

Specificity: THE^TM HA Tag Antibody, mAb, Mouse (A01244) recognizes C-terminal, N-terminal, and internal HA tagged-fusion proteins.

Characteristics: Anti-HA-tag mAb (ABIN387711) is a highly sensitive monoclonal antibody applicable to HA-tagged fusion protein detection. It belongs to our elite antibody collection. HA, or human influenza hemagglutinin, is a surface glycoprotein required for human influenza virus infectivity. The HA tag does not appear to interfere with the bioactivity or the biodistribution of recombinant proteins. Anti-HA-tag mAb (Antibodies-Online, ABIN387711) is a high-affinity monoclonal antibody that can be used to detect HA-tagged proteins. This product detects HA tags derived from an HA molecule corresponding to amino acids 98-106. It has been extensively used as a general epitope tag in expression vectors.

Purification: Protein A affinity column

Immunogen: A KLH coupled synthetic peptide (YPYDVPDYA)

Isotype: IgG1

Application Details

Application Notes: Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/mL
Western blot: 0.1-1.0 µg/mL
Immunoprecipitation: 1 µg/mL
Other applications: user-optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freezing and thawing cycles.

Storage: 4 °C/-20 °C

Storage Comment: The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below.

References for anti-HA-Tag antibody (ABIN387711)

Milewska, Kindler, Vkovski, Zeglen, Ochman, Thiel, Rajfur, Pyrc: "APOBEC3-mediated restriction of RNA virus replication." in: Scientific reports, Vol. 8, Issue 1, pp. 5960, (2019) (PubMed).

Naskalska, Dabrowska, Nowak, Szczepanski, Jasik, Milewska, Ochman, Zeglen, Rajfur, Pyrc: "Novel coronavirus-like particles targeting cells lining the respiratory tract." in: PLoS ONE, Vol. 13, Issue 9, pp. e0203489, (2019) (PubMed).

Cheng, Yu, Liu, Deng, Ma, Wang: "Functional analysis of bovine Nramp1 and production of transgenic cloned embryos in vitro." in: Zygote (Cambridge, England), Vol. 23, Issue 1, pp. 83-92, (2015) (PubMed).

Tanner, Liu, Oberste, Pallansch, Collett, Kirkegaard: "Dominant drug targets suppress the emergence of antiviral resistance." in: eLife, Vol. 3, (2014) (PubMed).

Pillay, Selland, Fleisch, Leighton, Cheng, Famulski, Ritzel, March, Wang, Allison, Waskiewicz: "Evaluating the mutagenic activity of targeted endonucleases containing a Sharkey FokI cleavage domain variant in zebrafish." in: Zebrafish, Vol. 10, Issue 3, pp. 353-64, (2013) (PubMed).

Shulga, Anderson, Topham, Epand: "Phosphatidylinositol-4-phosphate 5-kinase isoforms exhibit acyl chain selectivity for both substrate and lipid activator." in: The Journal of biological chemistry, Vol. 287, Issue 43, pp. 35953-63, (2012) (PubMed).

Borroto-Escuela, Romero-Fernandez, García-Negredo, Correia, Garriga, Fuxe, Ciruela: "Dissecting the conserved NPxxY motif of the M3 muscarinic acetylcholine receptor: critical role of Asp-7.49 for receptor signaling and multiprotein complex formation." in: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, Vol. 28, Issue 5, pp. 1009-22, (2011) (PubMed).

Target Details for HA-Tag

Target: HA-Tag

Alternative Name: HA Tag

Target Type: Tag