DNMT1 antibody (AA 1083-1112)

Catalog No. ABIN387879

This anti-DNMT1 antibody is a Rabbit Polyclonal antibody detecting DNMT1 in WB. Suitable for Human. This Primary Antibody has been cited in 4+ publications.

Quick Overview for DNMT1 antibody (AA 1083-1112) (ABIN387879)

Target: DNMT1 (DNA (Cytosine-5)-Methyltransferase 1 (DNMT1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DNMT1 antibody is un-conjugated

Application: Western Blotting (WB)

Clone: RB14236

Product Details anti-DNMT1 Antibody

Binding Specificity: AA 1083-1112

Predicted Reactivity: M, Rat

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This DNMT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1083-1112 amino acids from human DNMT1.

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

References for anti-DNMT1 antibody (ABIN387879)

Miousse, Murphy, Lin, Schisler, Sun, Chalbot, Sura, Johnson, LeBaron, Kavouras, Schnackenberg, Beger, Rasoulpour, Koturbash: "Dose-response analysis of epigenetic, metabolic, and apical endpoints after short-term exposure to experimental hepatotoxicants." in: Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association, Vol. 109, Issue Pt 1, pp. 690-702, (2018) (PubMed).

León-González, Jara-Palacios, Abbas, Heredia, Schini-Kerth: "Role of epigenetic regulation on the induction of apoptosis in Jurkat leukemia cells by white grape pomace rich in phenolic compounds." in: Food & function, Vol. 8, Issue 11, pp. 4062-4069, (2018) (PubMed).

Yin, Du, Cao, Liu, Xu, Xiang: "Reg3g Promotes Pancreatic Carcinogenesis in a Murine Model of Chronic Pancreatitis." in: Digestive diseases and sciences, (2015) (PubMed).

Chen, Wang, Hu, Chang, Liao, Dai, Juo: "OxLDL up-regulates microRNA-29b, leading to epigenetic modifications of MMP-2/MMP-9 genes: a novel mechanism for cardiovascular diseases." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 25, Issue 5, pp. 1718-28, (2011) (PubMed).

Target Details for DNMT1

Target: DNMT1 (DNA (Cytosine-5)-Methyltransferase 1 (DNMT1))

Alternative Name: DNMT1

Background: Methylation of DNA at cytosine residues plays an important role in regulation of gene expression, genomic imprinting and is essential for mammalian development. Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic DNA may be linked with development of cancer. To date, 3 families of mammalian DNA methyltransferase genes have been identified which include Dnmt1, Dnmt2 and Dnmt3. Dnmt1 is constitutively expressed in proliferating cells and inactivation of this gene causes global demethylation of genomic DNA and embryonic lethality. Dnmt2 is expressed at low levels in adult tissues and its inactivation does not affect DNA methylation or maintenance of methylation. The Dnmt3 family members, Dnmt3a and Dnmt3b, are strongly expressed in ES cells but their expression is down regulated in differentiating ES cells and is low in adult somatic tissue. Dnmt1 co-purifies with the retinoblastoma (Rb) tumour suppressor gene product, E2F1, and HDAC1. Dnmt1 also cooperates with Rb to repress transcription from promoters containing E2Fbinding sites suggesting a link between DNA methylation, histone deacetylase and sequence-specific DNA binding activity, as well as a growth-regulatory pathway that is disrupted in nearly all cancer cells.

Molecular Weight: 183165

Gene ID: 1786

NCBI Accession: NP_001124295, NP_001370

UniProt: P26358

Pathways: SARS-CoV-2 Protein Interactome, The Global Phosphorylation Landscape of SARS-CoV-2 Infection