KDM3A antibody (N-Term)

Catalog No. ABIN387995

The Rabbit Polyclonal anti-KDM3A antibody is suitable to detect KDM3A in samples from Human and Mouse. It has been validated for WB and IHC (p).

Quick Overview for KDM3A antibody (N-Term) (ABIN387995)

Target: KDM3A (Lysine (K)-Specific Demethylase 3A (KDM3A))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KDM3A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: RB14307

Product Details anti-KDM3A Antibody

Binding Specificity: AA 50-79, N-Term

Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogen: This JHDM2a antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 50-79 amino acids from the N-terminal region of human JHDM2a.

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:1000. IHC-P: 1:10~50

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

Target Details for KDM3A

Target: KDM3A (Lysine (K)-Specific Demethylase 3A (KDM3A))

Alternative Name: JHDM2a

Background: JHDM2a is a zinc finger protein that contains a jumonji domain. It is a histone demethylase that specifically demethylates 'Lys-9' of histone H3, thereby playing a central role in histone code. This protein preferentially demethylates mono- and dimethylated H3 'Lys-9' residue, with a preference for dimethylated residue, while it has weak or no activity on trimethylated H3 'Lys-9'. Demethylation of Lys residue generates formaldehyde and succinate. It is involved in hormone-dependent transcriptional activation, by participating in recruitment to androgen-receptor target genes, resulting in H3 'Lys-9' demethylation and transcriptional activation.

Molecular Weight: 147341

Gene ID: 55818

NCBI Accession: NP_001140160, NP_060903

UniProt: Q9Y4C1

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Nuclear Hormone Receptor Binding, Warburg Effect