GRP78 antibody

Catalog No. ABIN388133

There are 2+ publications for this product available. The Rabbit Polyclonal anti-GRP78 antibody is suitable to detect GRP78 in samples from Human and Mouse. It has been validated for WB, IF, IHC (p) and FACS.

Quick Overview for GRP78 antibody (ABIN388133)

Target: GRP78 (HSPA5) (Heat Shock 70kDa Protein 5 (Glucose-Regulated Protein, 78kDa) (HSPA5))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GRP78 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS)

Clone: RB1768

Product Details anti-GRP78 Antibody

Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogen: This HSPA5 antibody is generated from rabbits immunized with a recombinant protein encoding full length human HSPA5.

Isotype: Ig Fraction

Application Details

Application Notes: IF: 1:10~50. WB: 1:1000. WB: 1:1000. IHC-P: 1:50~100. IHC-P: 1:50~100. FC: 1:10~50

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots.

Expiry Date: 6 months

References for anti-GRP78 antibody (ABIN388133)

Ran, Mao, Shen, Xie, Zhan, Wang, Lu: "GRP78 enabled micelle-based glioma targeted drug delivery." in: Journal of controlled release : official journal of the Controlled Release Society, Vol. 255, pp. 120-131, (2018) (PubMed).

Buchkovich, Maguire, Paton, Paton, Alwine: "The endoplasmic reticulum chaperone BiP/GRP78 is important in the structure and function of the human cytomegalovirus assembly compartment." in: Journal of virology, Vol. 83, Issue 22, pp. 11421-8, (2009) (PubMed).

Target Details for GRP78

Target: GRP78 (HSPA5) (Heat Shock 70kDa Protein 5 (Glucose-Regulated Protein, 78kDa) (HSPA5))

Alternative Name: HSPA5

Background: In cooperation with other chaperones, HSP70s stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. The HSP70s in mitochondria and the endoplasmic reticulum play an additional role by providing a driving force for protein translocation. They are involved in signal transduction pathways in cooperation with HSP90. They participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage.

Molecular Weight: 72333

Gene ID: 3309

NCBI Accession: NP_005338

UniProt: P11021

Pathways: Thyroid Hormone Synthesis, ER-Nucleus Signaling