ATG5 antibody (C-Term)

Catalog No. ABIN388519

This Rabbit Polyclonal antibody specifically detects ATG5 in WB, IF and IHC (p). It exhibits reactivity toward Human and Mouse and has been mentioned in 29+ publications.

Quick Overview for ATG5 antibody (C-Term) (ABIN388519)

Target: ATG5 (ATG5 Autophagy Related 5 (ATG5))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATG5 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: RB7547

Product Details anti-ATG5 Antibody

Binding Specificity: AA 209-238, C-Term

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This ATG5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 209-238 amino acids from the C-terminal region of human ATG5.

Isotype: IgG

Application Details

Application Notes: IF: 1:25. WB: 1:1000. WB: 1:1000. WB: 1:1000. IHC-P: 1:50~100

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots.

Expiry Date: 6 months

References for anti-ATG5 antibody (ABIN388519)

Zhang, Michel, Lenz, Friedel, Köster, dHedouville, Tönges, Urlaub, Bähr, Lingor, Koch: "Calpain-mediated cleavage of collapsin response mediator protein-2 drives acute axonal degeneration." in: Scientific reports, Vol. 6, pp. 37050, (2018) (PubMed).

Radogna, Cerella, Gaigneaux, Christov, Dicato, Diederich: "Cell type-dependent ROS and mitophagy response leads to apoptosis or necroptosis in neuroblastoma." in: Oncogene, Vol. 35, Issue 29, pp. 3839-53, (2017) (PubMed).

Kessel, Evans: "Promotion of Proapoptotic Signals by Lysosomal Photodamage: Mechanistic Aspects and Influence of Autophagy." in: Photochemistry and photobiology, Vol. 92, Issue 4, pp. 620-3, (2017) (PubMed).

Zhang, Gao, Zhang, Song, Cheng, Zhou: "The germline-enriched Ppp1r36 promotes autophagy." in: Scientific reports, Vol. 6, pp. 24609, (2017) (PubMed).

Mathai, Meijer, Simonsen: "Studying Autophagy in Zebrafish." in: Cells, Vol. 6, Issue 3, (2017) (PubMed).

Du, Ren, Yao, Duan, Zhao, Du, Xiao, Duan, Shi: "Sphingosine kinase 1 protects renal tubular epithelial cells from renal fibrosis via induction of autophagy." in: The international journal of biochemistry & cell biology, Vol. 90, pp. 17-28, (2017) (PubMed).

Paquet, Nicoll, Love, Mouton-Liger, Holmes, Hugon, Boche: "Downregulated apoptosis and autophagy after anti-Aβ immunotherapy in Alzheimer's disease." in: Brain pathology (Zurich, Switzerland), (2017) (PubMed).

Wang, Li, Wang, Ma, Chuai, Cao, Yang: "High glucose environment inhibits cranial neural crest survival by activating excessive autophagy in the chick embryo." in: Scientific reports, Vol. 5, pp. 18321, (2016) (PubMed).

Varga, Fodor, Vellai: "Autophagy in zebrafish." in: Methods (San Diego, Calif.), Vol. 75, pp. 172-80, (2015) (PubMed).

Ribas, Schnepf, Challagundla, Koch, Bähr, Lingor: "Early and sustained activation of autophagy in degenerating axons after spinal cord injury." in: Brain pathology (Zurich, Switzerland), Vol. 25, Issue 2, pp. 157-70, (2015) (PubMed).

Hsin, Ou, Wu, Jan, Hsiao, Lin, Ko: "GMI, an Immunomodulatory Protein from Ganoderma microsporum, Potentiates Cisplatin-Induced Apoptosis via Autophagy in Lung Cancer Cells." in: Molecular pharmaceutics, Vol. 12, Issue 5, pp. 1534-43, (2015) (PubMed).

Xin, Xiao, Ma, He, Yao, Liu et al.: "Coxsackievirus B3 induces crosstalk between autophagy and apoptosis to benefit its release after replicating in autophagosomes through a mechanism involving caspase cleavage of autophagy-related ..." in: Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases, Vol. 26, pp. 95-102, (2014) (PubMed).

Degtyarev, Reichelt, Lin: "Novel quantitative autophagy analysis by organelle flow cytometry after cell sonication." in: PLoS ONE, Vol. 9, Issue 1, pp. e87707, (2014) (PubMed).

Yao, Jia, Shelby, Ganios, Feathers, Thompson, Zacks: "Circadian and noncircadian modulation of autophagy in photoreceptors and retinal pigment epithelium." in: Investigative ophthalmology & visual science, Vol. 55, Issue 5, pp. 3237-46, (2014) (PubMed).

Al-Zeer, Al-Younes, Lauster, Abu Lubad, Meyer: "Autophagy restricts Chlamydia trachomatis growth in human macrophages via IFNG-inducible guanylate binding proteins." in: Autophagy, Vol. 9, Issue 1, pp. 50-62, (2013) (PubMed).

Zhang, Morgan, Chen, Choksi, Liu: "Induction of autophagy is essential for monocyte-macrophage differentiation." in: Blood, Vol. 119, Issue 12, pp. 2895-905, (2012) (PubMed).

Chiu, Chen, Ho, Wang: "Arsenic trioxide enhances the radiation sensitivity of androgen-dependent and -independent human prostate cancer cells." in: PLoS ONE, Vol. 7, Issue 2, pp. e31579, (2012) (PubMed).

Hu, Zhang, Zhang: "Expression pattern and functions of autophagy-related gene atg5 in zebrafish organogenesis." in: Autophagy, Vol. 7, Issue 12, pp. 1514-27, (2012) (PubMed).

Norberg, Karlsson, Korenovska, Szydlowski, Silberberg, Uhlén, Orrenius, Zhivotovsky: "Critical role for hyperpolarization-activated cyclic nucleotide-gated channel 2 in the AIF-mediated apoptosis." in: The EMBO journal, Vol. 29, Issue 22, pp. 3869-78, (2010) (PubMed).

Dou, Chattopadhyay, Pan, Guerriero, Jiang, Ballou, Yue, Lin, Zong: "The class IA phosphatidylinositol 3-kinase p110-beta subunit is a positive regulator of autophagy." in: The Journal of cell biology, Vol. 191, Issue 4, pp. 827-43, (2010) (PubMed).

Target Details for ATG5

Target: ATG5 (ATG5 Autophagy Related 5 (ATG5))

Alternative Name: ATG5

Background: Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). APG5, required for autophagy, conjugates to ATG12 and associates with an isolation membrane to form a cup-shaped isolation membrane and autophagosome. The conjugate detaches from the membrane immediately before or after autophagosome formation is completed. APG5 may also play an important role in the apoptotic process, possibly within the modified cytoskeleton. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity.

Molecular Weight: 32447

Gene ID: 9474

NCBI Accession: NP_004840

UniProt: Q9H1Y0

Pathways: Activation of Innate immune Response, Production of Molecular Mediator of Immune Response, Autophagy