PROX1 antibody (AA 185-214)

Catalog No. ABIN388777

This Rabbit Polyclonal antibody specifically detects PROX1 in WB and IHC (p). It exhibits reactivity toward Human and Mouse.

Quick Overview for PROX1 antibody (AA 185-214) (ABIN388777)

Target: PROX1 (Prospero Homeobox 1 (PROX1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PROX1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: RB13371

Product Details anti-PROX1 Antibody

Binding Specificity: AA 185-214

Predicted Reactivity: M

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This PROX1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 185-214 amino acids from the Central region of human PROX1.

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:500. WB: 1:500. IHC-P: 1:10~50

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

Target Details for PROX1

Target: PROX1 (Prospero Homeobox 1 (PROX1))

Alternative Name: PROX1

Background: The expression pattern of Prox1 suggests that it has a role in a variety of embryonic tissues, including lens. Prox mRNA is present in many different human tissues with lens demonstrating the highest level. Homozygous Prox1-null mice die at midgestation from multiple developmental defects, and a targeted effect on lens development has been reported. Prox1 inactivation caused abnormal cellular proliferation, downregulated expression of the cell cycle inhibitors Cdkn1b and Cdkn1c, misexpression of E-cadherin, and excessive apoptosis. Consequently, mutant lens cells failed to polarize and elongate properly, resulting in a hollow lens. Prox1 is expressed in a subpopulation of endothelial cells that by budding and sprouting give rise to the lymphatic system. Prox1 appears to be a specific and required regulator of the development of the lymphatic system. Prox1 also has been documented to be required for hepatocyte migration in the mouse. Loss of Prox1 results in a smaller liver with a reduced population of clustered hepatocytes. The homeodomain protein Prox1 regulates the egress of progenitor cells from the cell cycle in the embryonic mouse retina. Cells lacking Prox1 are less likely to stop dividing, and ectopic expression of Prox1 forces progenitor cells to exit the cell cycle. Prox1 acts as a key participant in progenitor-cell proliferation and cell-fate determination in the vertebrate retina.

Molecular Weight: 83203

Gene ID: 5629

NCBI Accession: NP_001257545, NP_002754

UniProt: Q92786

Pathways: Stem Cell Maintenance, Regulation of Muscle Cell Differentiation