ARL3 antibody (N-Term)

Catalog No. ABIN389000

The Rabbit Polyclonal anti-ARL3 antibody has been validated for WB. It is suitable to detect ARL3 in samples from Human.

Quick Overview for ARL3 antibody (N-Term) (ABIN389000)

Target: ARL3 (ADP-Ribosylation Factor-Like 3 (ARL3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ARL3 antibody is un-conjugated

Application: Western Blotting (WB)

Clone: RB4909-4910

Product Details anti-ARL3 Antibody

Binding Specificity: AA 1-30, N-Term

Predicted Reactivity: B, M, Pig, Rat, X

Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogen: This ARL3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human ARL3.

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

Target Details for ARL3

Target: ARL3 (ADP-Ribosylation Factor-Like 3 (ARL3))

Alternative Name: ARL3

Background: ADP-ribosylation factors (ARFs) are low molecular weight GTP-binding proteins belonging to the RAS superfamily. The predicted 182-amino acid ARL3 (ADP-ribosylation like factor) protein shares 97 % amino acid identity with rat ARLl3 and 43 % identity with human ARF1. Like the ARFs, ARL3 has a glycine at position 2, the site of N myristoylation, and lacks cysteine residues near the C terminus, which are found in other members of the RAS family. Northern blot analysis detected a 1-kb ARL3 transcript in all tissues tested, with highest expression in heart and lung, and lower expression in brain, liver, kidney, ovary, and testis. A 5.5-kb transcript was also detected in most tissues, with highest expression in brain. Immunoblot analysis detected ARL3 in human tumor cell lines but not in normal rodent cells. Although ARL3 binds GTP, it is devoid of activity in the cholera toxin-dependent ADP-ribosylation of Gs, and is therefore classified as an ARF-like protein.

Molecular Weight: 20456

Gene ID: 403

NCBI Accession: NP_004302

UniProt: P36405