GPLD1 antibody (C-Term)

Catalog No. ABIN389063

The Rabbit Polyclonal anti-GPLD1 antibody is suitable to detect GPLD1 in samples from Human. It has been validated for WB and IHC (p).

Quick Overview for GPLD1 antibody (C-Term) (ABIN389063)

Target: GPLD1 (Glycosylphosphatidylinositol Specific phospholipase D1 (GPLD1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GPLD1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: RB04854

Product Details anti-GPLD1 Antibody

Binding Specificity: AA 679-709, C-Term

Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogen: This GPLD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 679-709 amino acids from the C-terminal region of human GPLD1.

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:1000. IHC-P: 1:50~100

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

Target Details for GPLD1

Target: GPLD1 (Glycosylphosphatidylinositol Specific phospholipase D1 (GPLD1))

Alternative Name: GPLD1

Background: GPLD1 is expressed in numerous tissues and cells and specifically cleaves GPI-anchored proteins. Liver has the highest level of GPI-PLD expression and is the primary organ contributing to GPLD1 in the serum. GPLD1 is abundant in serum in which it associates with polipoproteins AI and AIV. Increased serum GPLD1 is associated with insulin resistance and elevated serum triglycerides. Many surface proteins are attached to eukaryotic cell membranes via glycosylphosphatidylinositol (GPI) anchors that are covalently bound to the C-terminus of the protein and cleavage of the GPI moiety by GPLD1, only enzyme known that cleavage GPI anchor, may represent a means of regulating attachment of these proteins to the cell surface, or alternatively, their release into the extracellular environment.

Molecular Weight: 92336

Gene ID: 2822

NCBI Accession: NP_001494

UniProt: P80108

Pathways: Inositol Metabolic Process, Regulation of Carbohydrate Metabolic Process