PHGDH antibody (N-Term)

Catalog No. ABIN389483

The Rabbit Polyclonal anti-PHGDH antibody is suitable to detect PHGDH in samples from Human and Mouse. It has been validated for WB, FACS and IHC (p).

Quick Overview for PHGDH antibody (N-Term) (ABIN389483)

Target: PHGDH (phosphoglycerate Dehydrogenase (PHGDH))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PHGDH antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: RB20882

Product Details anti-PHGDH Antibody

Binding Specificity: AA 140-168, N-Term

Predicted Reactivity: B, Pr, Pig

Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogen: This PHGDH antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 140-168 amino acids from the N-terminal region of human PHGDH.

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:1000. WB: 1:1000. IHC-P: 1:50~100. FC: 1:10~50

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

Target Details for PHGDH

Target: PHGDH (phosphoglycerate Dehydrogenase (PHGDH))

Alternative Name: PHGDH

Background: 3-Phosphoglycerate dehydrogenase catalyzes the transition of 3-phosphoglycerate into 3-phosphohydroxypyruvate, which is the first and rate-limiting step in the phosphorylated pathway of serine biosynthesis, using NAD+/NADH as a cofactor.

Molecular Weight: 56651

Gene ID: 26227

NCBI Accession: NP_006614

UniProt: O43175

Pathways: Metabolism of Steroid Hormones and Vitamin D, Warburg Effect