HIST1H3B3 (pSer10) antibody

Catalog No. ABIN389570

The Rabbit Polyclonal anti-HIST1H3B3 antibody is suitable to detect HIST1H3B3 in samples from Human. It has been validated for DB, WB, IF and IHC (p).

Quick Overview for HIST1H3B3 (pSer10) antibody (ABIN389570)

Target: HIST1H3B3

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Dot Blot (DB), Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: RB2673-RB2674

Product Details

Binding Specificity: pSer10

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This HIST1H3B3 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S10 of human HIST1H3B3.

Isotype: Ig Fraction

Application Details

Application Notes: IF: 1:25. WB: 1:500. WB: 1:500. IHC-P-Leica: 1:100. IHC-P-Leica: 1:100. DB: 1:500

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots.

Expiry Date: 6 months

Target Details

Target: HIST1H3B3

Background: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails, instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3.

Molecular Weight: 15404

Gene ID: 8968

NCBI Accession: NP_003520, NP_003521, NP_003522, NP_003523, NP_003524, NP_003525, NP_003526, NP_003527, NP_003528, NP_066298

UniProt: P68431