SMAD3 antibody (pSer208)

Catalog No. ABIN389656

This Rabbit Polyclonal antibody specifically detects SMAD3 in IF and DB. It exhibits reactivity toward Humanand has been mentioned in 1 publication.

Quick Overview for SMAD3 antibody (pSer208) (ABIN389656)

Target: SMAD3 (SMAD, Mothers Against DPP Homolog 3 (SMAD3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SMAD3 antibody is un-conjugated

Application: Immunofluorescence (IF), Dot Blot (DB)

Clone: RB31592-RB31594

Product Details anti-SMAD3 Antibody

Binding Specificity: pSer208

Predicted Reactivity: C, M, Pig, Rat

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This SMAD3 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S208 of human SMAD3.

Isotype: Ig Fraction

Application Details

Application Notes: IF: 1:200. IF: 1:10~50. DB: 1:500

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots.

Expiry Date: 6 months

References for anti-SMAD3 antibody (ABIN389656)

Cohen-Solal, Merrigan, Chan, Goydos, Chen, Foran, Liu, Lasfar, Reiss: "Constitutive Smad linker phosphorylation in melanoma: a mechanism of resistance to transforming growth factor-?-mediated growth inhibition." in: Pigment cell & melanoma research, Vol. 24, Issue 3, pp. 512-24, (2011) (PubMed).

Target Details for SMAD3

Target: SMAD3 (SMAD, Mothers Against DPP Homolog 3 (SMAD3))

Alternative Name: SMAD3

Background: SMAD3, a receptor regulated SMAD (R-SMAD) is a transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinase. SMAD3 is estimated to account for at least 80 % of all TGF-beta-mediated response. Activated type I receptor phosphorylates receptor-activated SMADS (RSMADS) at their c-terminal two extreme serines in the SSXS motif. The phosphorylated R-SMAD translocate into nucleus, where they regulate transcription of target genes. SMAD3 signal transduction appears to be important in the rgulation of muscle-specific genes. Loss of SMAD3 is a feature of pediatric T-cell lymphoblastic leukemia, while upregulation of SMAD3 may be responsible for TGFB hyperresponsiveness observed in scleroderma.

Molecular Weight: 48081

Gene ID: 4088

NCBI Accession: NP_001138574, NP_001138575, NP_001138576, NP_005893

UniProt: P84022

Pathways: Cell Division Cycle, Chromatin Binding, Cell-Cell Junction Organization, Positive Regulation of Endopeptidase Activity, Autophagy