Cathepsin F antibody (AA 261-290)

Catalog No. ABIN390488

The Rabbit Polyclonal anti-Cathepsin F antibody is suitable to detect Cathepsin F in samples from Human. It has been validated for WB, FACS and IHC (p).

Quick Overview for Cathepsin F antibody (AA 261-290) (ABIN390488)

Target: Cathepsin F (CTSF)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cathepsin F antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: RB19248

Product Details anti-Cathepsin F Antibody

Binding Specificity: AA 261-290

Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogen: This CTSF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 261-290 amino acids from the Central region of human CTSF.

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:1000. IHC-P: 1:50~100. FC: 1:10~50

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

Target Details for Cathepsin F

Target: Cathepsin F (CTSF)

Alternative Name: CTSF

Background: Cathepsins are papain family cysteine proteinases that represent a major component of the lysosomal proteolytic system. Cathepsins generally contain a signal sequence, followed by a propeptide and then a catalytically active mature region. The very long (251 amino acid residues) proregion of the cathepsin F precursor contains a C-terminal domain similar to the pro-segment of cathepsin L-like enzymes, a 50-residue flexible linker peptide, and an N-terminal domain predicted to adopt a cystatin-like fold. The cathepsin F proregion is unique within the papain family cysteine proteases in that it contains this additional N-terminal segment predicted to share structural similarities with cysteine protease inhibitors of the cystatin superfamily. This cystatin-like domain contains some of the elements known to be important for inhibitory activity. CTSF is a predicted protein of 484 amino acids which contains a 19 residue signal peptide. Cathepsin F contains five potential N-glycosylation sites, and it may be targeted to the endosomal/lysosomal compartment via the mannose 6-phosphate receptor pathway.

Molecular Weight: 53366

Gene ID: 8722

NCBI Accession: NP_003784

UniProt: Q9UBX1