PKM2 antibody (N-Term)

Catalog No. ABIN391052

The Rabbit Polyclonal anti-PKM2 antibody has been validated for WB. It is suitable to detect PKM2 in samples from Human and Mouse. There is 1 publication available.

Quick Overview for PKM2 antibody (N-Term) (ABIN391052)

Target: PKM2 (Pyruvate Kinase M2 (PKM2))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PKM2 antibody is un-conjugated

Application: Western Blotting (WB)

Clone: RB5870

Product Details anti-PKM2 Antibody

Binding Specificity: AA 121-151, N-Term

Predicted Reactivity: Rb, Rat

Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogen: This Pyruvate Kinase (PKM2) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 121-151 amino acids from the N-terminal region of human Pyruvate Kinase (PKM2).

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:2000. WB: 1:2000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

References for anti-PKM2 antibody (ABIN391052)

Shin, Yoo, Hong, Chang, Jung, Jeong, Park: "Upregulation of glycolytic enzymes in proteins secreted from human colon cancer cells with 5-fluorouracil resistance." in: Electrophoresis, Vol. 30, Issue 12, pp. 2182-92, (2009) (PubMed).

Target Details for PKM2

Target: PKM2 (Pyruvate Kinase M2 (PKM2))

Alternative Name: Pyruvate Kinase (PKM2)

Background: PKM2 is a pyruvate kinase that catalyzes the production of phosphoenolpyruvate from pyruvate and ATP. This protein has been shown to interact with thyroid hormone, and thus may mediate cellular metabolic effects induced by thyroid hormones. This protein has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis.

Molecular Weight: 57937

Gene ID: 5315

NCBI Accession: NP_001193725, NP_001193726, NP_001193727, NP_001193728, NP_002645, NP_872270, NP_872271

UniProt: P14618

Pathways: Warburg Effect