ILK antibody (C-Term)

Catalog No. ABIN391105

This Rabbit Polyclonal antibody specifically detects ILK in WB. It exhibits reactivity toward Human and Mouse.

Quick Overview for ILK antibody (C-Term) (ABIN391105)

Target: ILK (Integrin-Linked Kinase (ILK))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ILK antibody is un-conjugated

Application: Western Blotting (WB)

Clone: RB5343

Product Details anti-ILK Antibody

Binding Specificity: AA 391-421, C-Term

Predicted Reactivity: B, Rat

Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogen: This ILK2/ILK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 391-421 amino acids from the C-terminal region of human ILK2/ILK1.

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:1000. WB: 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

Target Details for ILK

Target: ILK (Integrin-Linked Kinase (ILK))

Alternative Name: ILK2/ILK1

Background: Transduction of extracellular matrix signals through integrins influences intracellular and extracellular functions, and appears to require interaction of integrin cytoplasmic domains with cellular proteins. Integrin-linked kinase (ILK) is an ankyrin repeat containing 51 kDa receptor-proximate serine-threonine kinase (1), with a reported migration rate of 59K. This 451 amino acid protein interacts with the cytoplasmic domain of the beta-1 integrin subunit and contains sequence motifs found in pleckstrin homology domains capable of interacting with phosphoinositide lipids. ILK is an upstream regulator of Pi(3)K dependant activation of protein kinase B (PKB/AKT) and inhibition of glycogen synthase kinase 3 (GSK-3). ILK2 expression is associated with mediation of cell architecture, adhesion to integrin substrates and anchorage-dependent growth in epithelial cells. ILK2 is overexpressed in some highly invasive tumor cell lines.

Molecular Weight: 51419

Gene ID: 3611

NCBI Accession: NP_001014794, NP_001014795, NP_001265370, NP_004508

UniProt: Q13418

Pathways: Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Maintenance of Protein Location, Skeletal Muscle Fiber Development, Smooth Muscle Cell Migration