SMAD4 antibody (AA 255-284)

Catalog No. ABIN392165

The Rabbit Polyclonal anti-SMAD4 antibody has been validated for WB and IF. It is suitable to detect SMAD4 in samples from Human and Mouse. There is 1 publication available.

Quick Overview for SMAD4 antibody (AA 255-284) (ABIN392165)

Target: SMAD4 (SMAD Family Member 4 (SMAD4))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SMAD4 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Clone: RB14714

Product Details anti-SMAD4 Antibody

Binding Specificity: AA 255-284

Predicted Reactivity: Rat

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This SMAD4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 255-284 amino acids from human SMAD4.

Isotype: Ig Fraction

Application Details

Application Notes: IF: 1:10~50. WB: 1:1000. WB: 1:2000. WB: 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

References for anti-SMAD4 antibody (ABIN392165)

Lessard, Rivas, Alves-Wagner, Hirshman, Gallagher, Constantin-Teodosiu, Atkins, Greenhaff, Qi, Gustafsson, Fielding, Timmons, Britton, Koch, Goodyear: "Resistance to aerobic exercise training causes metabolic dysfunction and reveals novel exercise-regulated signaling networks." in: Diabetes, Vol. 62, Issue 8, pp. 2717-27, (2013) (PubMed).

Target Details for SMAD4

Target: SMAD4 (SMAD Family Member 4 (SMAD4))

Alternative Name: SMAD4

Background: SMAD4 is the common SMAD (co-SMAD)mediator of signal transduction by TGF-beta (transforming growth factor). It promotes binding of the SMAD2/SMAD4/FAST-1 complex to DNA and provides an activation function required for SMAD1 or SMAD2 to stimulate transcription. It may act as a tumor suppressor.

Molecular Weight: 60439

Gene ID: 4089

NCBI Accession: NP_005350

UniProt: Q13485

Pathways: Cell Division Cycle, Chromatin Binding, Autophagy