DGKQ antibody (C-Term)

Catalog No. ABIN392717

The Rabbit Polyclonal anti-DGKQ antibody is suitable to detect DGKQ in samples from Human. It has been validated for WB, IHC (p) and FACS.

Quick Overview for DGKQ antibody (C-Term) (ABIN392717)

Target: DGKQ (Diacylglycerol Kinase, theta 110kDa (DGKQ))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DGKQ antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS)

Clone: RB3823

Product Details anti-DGKQ Antibody

Binding Specificity: AA 912-942, C-Term

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This DGKQ antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 912-942 amino acids from the C-terminal region of human DGKQ.

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:1000. IHC-P: 1:10~50. FC: 1:10~50

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

Target Details for DGKQ

Target: DGKQ (Diacylglycerol Kinase, theta 110kDa (DGKQ))

Alternative Name: DGKQ

Background: Diacylglycerol (DAG) is an allosteric activator of protein kinase C. DAG also participates in regulating RAS and RHO family proteins by activating the guanine nucleotide exchange factors VAV and RASGRP1. DAG is also involved in the synthesis of phospholipids and triacylglycerols. Tight regulation of DAG levels is achieved via DAG kinases (DGKs), which remove DAG by phosphorylate it to phosphatidic acid.The predicted 882-amino acid human DGKQ (DGK-theta) protein is 90 % identical in sequence to the rat homolog. DGK-theta is comprised of a pleckstrin homology domain and the conserved DGK putative catalytic domain. In contrast to other DGK isotypes, DGK-theta contains 3 rather than 2 cysteine-rich zinc-binding domains, an N-terminal proline- and glycine-rich region, and a RAS-associating domain. Highest tissue expression in the rat is in the brain. Defects in eye-specific DAGK genes cause retinal degeneration in Drosophila, thus, DAGK genes are candidates for human eye disease.

Molecular Weight: 101155

Gene ID: 1609

NCBI Accession: NP_001338

UniProt: P52824